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To measure 5′-nucleotidase activity, this procedure uses adenosine 5’-monophosphate and a color reagent to create a standard curve for determining the micromoles of phosphorus liberated.

Order shRNA Online in 5 Easy Steps

5 easy steps to order shRNA gene of your interest.

5'/3' RACE Kit, 2nd Generation Troubleshooting

In addition to the troubleshooting provided in the product manual, most probably the efficiency of the tailing reaction performed by Terminal Transferase could be impaired. This could occur due to several reasons (which will not only affect the control reaction

The 3'/5' Assay for Analysis of RNA Integrity Protocol

The 3’/5’ integrity assay identifies RNA degradation, crucial for large sample sets or less detectable degradation.

Cell Culture Protocol 5: Subculture of Suspension Cell Lines

Cell culture protocol for passaging and splitting suspension cell lines

Download our Training Program

Our Training Program is availabale in 5 different languages.

Puriﬁcation using StrepTrap™ HP 1 mL and 5 mL

This page shows how to purify Strep-tag II recombinant proteins using StrepTrap HP 1 mL and 5 mL from Cytiva.

Custom Peptides

Peptide synthesis up to 200 amino acids with HPLC purification, offering various purity levels, scales, and modifications.

Hydroxymethylfurfural Analysis of Beer by HPLC UV

HPLC-UV determination of 5-HMF (5-(hydroxymethyl)furfural) in beer samples using monolithic silica Chromolith® HighResolution RP-18e HPLC column.

FastStart™ Taq DNA Polymerase, 5 U/μL Protocol & Troubleshooting

The choice of the PCR enzyme in combination with an appropriate buffer can profoundly affect PCR outcome.

Custom Oligos Modifications

We offer over 200 custom DNA oligos modification for both DNA, RNA oligonucleotides and sequencing to probes for gene.

Merck Opens Climate-Neutral €150 Million Filter Manufacturing Facility in Ireland

Merck opens first climate-neutral manufacturing facility in Ireland. €150M investment creates 200+ jobs in filter production.

Biglycan

Biglycan is a 200-350 kDa(proteoglycan consisting of a 45 kDa core protein and two chrondroitin/dermatan sulfate glycosaminoglycan chains.

5 Reasons Cancer Researchers Adopt 3D Cell Culture: A Review of Recent Literature

Numerous cancer model systems are available to investigate disease mechanisms and to screen therapies. While all of the models have contributed critical information about cancer biology.

Signal Peptide Optimization: Effect On Recombinant Monoclonal IgG Productivity, Product Quality And Antigen-Binding Affinity

A signal peptide is a 5-30 amino acid (aa) peptide present at the N-terminus of secretory proteins.

Reflectometric Determination of Hydroxymethylfurfural in Honey

Rapid analysis of 5-(Hydroxymethyl)furfural (HMF) in honey using a reflectometer and Reflectoquant® HMF Test.

Critical Inhibitor Characteristics to Consider

Discover critical characteristics to consider when working with enzyme inhibitors, such as cell permeability, the prozone effect, and Lipinski’s rule of 5.

Sodium acetate buffer solution for molecular biology

Sodium Acetate Buffer provider. 5+ decades providing high-quality buffer products that maintain consistent performance for molecular biology .

Review of O-Linked Glycoproteins

O-Linked glycoproteins are usually large proteins with a molecular mass of >200 kDa. Glycosylation generally occurs in high-density clusters and may represent as much as 50-80% of the overall mass.

Block Copolymers in Nanoscale Patterning

Block copolymers coat surfaces for nanoscale patterns, improving resolution to 5-50nm, enabling diverse applications like printable circuits.

Luminex^® Multiplexing Platforms Comparison

Uncover which system is right for your research by comparing the Luminex® multiplexing platforms including the Luminex® 200™, FLEXMAP 3D®, and xMAP® INTELLIFLEX® platforms.

Ethanol

We offer a number of ethanol products suitable for applications including HPLC, GC, and molecular biology. Our offerings include 190 proof ethanol, 200 proof absolute ethanol, and denatured ethanol.

Universal ProbeLibrary System Technology

UPL is based on only 165 short hydrolysis probes. They are labeled at the 5' end with fluorescein (FAM) and at the 3' end with a dark quencher dye.

Counterstaining after the Duolink In Situ Protocol

We recommend applying the counterstaining protocol after the completion of the Amplification step in section 7.3, step 5 of the Duolink In Situ Fluorescence User Manual.

Integrity Testers

Efficient Filter Integrity Testing: Elevate quality control with Integritest® 5, offering bubble point, diffusion, and hydrophobic testing in-line or offline.

Calf Intestinal (CIP) Alkaline Phosphatase for Nucleic Acid Dephosphorylation

CIP is used to remove 5’-phosphate groups from DNA, RNA and both ribo and deoxy-ribonucleoside triphosphates. Detailed protocol on how to dephosphorylate DNA.

QuEChERS Method for Pesticide Residue Analysis

QuEChERS method for pesticide residue analysis requires only small quantities of solvent and is capable of generating recoveries of 70-120% with RSDs <5% for a wide range of compounds.

Digoxigenin (DIG) Labeling Methods

Digoxigenin (DIG) labeling methods and kits for DNA and RNA DIG probes, random primed DNA labeling, nick translation labeling, 5’ and 3’ oligonucleotide end-labeling.

Critical Micelle Concentrations (CMCs) - Avanti® Polar Lipids

The critical micelle concentration (CMC) can be approximately defined as the lipid monomer concentration at which appreciable amounts (>5% of total) of micellar aggregates first begin to appear in the equilibrium: nM1Mn

Non-Fat Dry Milk (NFDM) Solution for Blocking Western Blots

Non-Fat Dry milk (NFDM) buffered solution is ideal for saturating excess protein-binding sites on membranes for Western blotting. Typically, 5% NFDM is sufficient for most Western blots.

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