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200-675-3
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3D Insert Cell Seeding Protocol
After 3 h Total Volume (µl) After 3 h Scaffold
Size PS1520 PS3040 PCL3030 PCL3050 PS1520 PS3040 PCL3030 PCL3050
96‐well 185 180 180 175 200 200 200 200
48‐well 225 200 200 180
Product Information Sheet - CS0010
Precursor Protein 770 Fragment 662-675. The inhibition profile of purified BACE1 activity by this
inhibitor is described in Figure 2.
Reactions 5-8: standard curve
3
4. Read
3D Biotek Cell Seeding Flow-Chart
further support, please visit us at http://www.3DBiotek.com
Technology Center of New Jersey ■ 675 US Highway One, North Brunswick, New Jersey 08902, USA ■ E-mail: [email protected]
Version 3.0
Data Sheet - K0515
Anti- KV12.1 (Elk1) antibody, 1:200.
Lane 2. Anti- KV12.1 (Elk1) antibody, preincubated with
the control peptide antigen
References
1. Shi, W. et al. J. Physiol. 511, 675-682 (1998).
2. Zou, A. et al.
Data Sheet-SCT085
Spectral Properties
Excitation: 650 nm
Emission: 675 nm
Figure 1: Probe absorbance data. 7 µL of probe at
stock concentration (200 µM) was diluted in 1 mL of
DMSO before undergoing
Product Information Sheet - MAK142
at λex = 640/λem = 675 nm
using either a fluorescence microplate reader, flow
cytometer, or a fluorescent microscope with Cy5 filter.
Components
The kit is sufficient for 200 assays in 96 well plates
Data Sheet - N7404
of
niacin
(0, 25, 50, 75, 125, 150, 200, 250 ng/tube (10 ml);
turbidimetric readings at 620 nm).
References:
1. Snell and Wright, J. Biol. Chem., 13, 675 (1941)
2. Krehl, Strong and Elvetjam
Enzymatic Assay of L-AMINO ACID OXIDASE
Enzymology, XVII B, 593-596
Knox, W. E. and Pitt, B. M. (1957) Journal of Biological Chemistry 225, 675-688
NOTES:
1. Catalase Unit Definition: One unit will decompose 1.0 PROH�RI�+2O2 per minute
Enzymatic Assay of L-AMINO ACID OXIDASE
Enzymology, XVII B, 593-596
Knox, W. E. and Pitt, B. M. (1957) Journal of Biological Chemistry 225, 675-688
NOTES:
1. Catalase Unit Definition: One unit will decompose 1.0 PROH�RI�+2O2 per minute
L -Amino Acid Oxidase from Bothrops atrox venom (A4257)
Enzymology, XVII B, 593-596
Knox, W. E. and Pitt, B. M. (1957) Journal of Biological Chemistry 225, 675-688
NOTES:
1. Catalase Unit Definition: One unit will decompose 1.0 PROH�RI�+2O2 per minute
L -Amino Acid Oxidase from Crotalus durissus venom (A2805)
Enzymology, XVII B, 593-596
Knox, W. E. and Pitt, B. M. (1957) Journal of Biological Chemistry 225, 675-688
NOTES:
1. Catalase Unit Definition: One unit will decompose 1.0 PROH�RI�+2O2 per minute
Data Sheet - T1830 - Lot 109K0797
5-fold with a 50 ng/µl BSA solution.
0
50,000
100,000
150,000
200,000
0 225 450 675 900
Protein (ng)
A
ct
iv
ity
(c
pm
)
Data Sheet - SRP5092
5-fold with a 50 ng/µl BSA solution.
0
50,000
100,000
150,000
200,000
0 225 450 675 900
Protein (ng)
A
ct
iv
ity
(C
P
M
)
Data Sheet - T1830 - Lot SLBB2534
5-fold with a 50 ng/µl BSA solution.
0
50,000
100,000
150,000
200,000
0 225 450 675 900
Protein (ng)
A
ct
iv
ity
(c
pm
)
Data Sheet - P9124
Prokocimer, M., et al., Hum. Mutat., 12, 4-18
(1998).
15. Shaw, P.H., Pathol. Res. Pract., 192, 669-675
(1996).
16. Shieh, S.Y., et al., Cell, 91, 325-334 (1997).
EK,KAA,PHC 09/06-1
Sigma brand
Data Sheet - SAB4600092
Figure 1).
Figure 1.
Absorption/Emission Spectra of CF594 Conjugated
Antibodies
475 525 575 625 675 725
Wavelength (nm)
A
bs
or
pt
io
n E
m
ission
λabs = 593 nm (PBS buffer, pH 7.4)
λem
Data Sheet - SAB4600102
Figure 1).
Figure 1.
Absorption/Emission Spectra of CF594 Conjugated
Antibodies
475 525 575 625 675 725
Wavelength (nm)
A
bs
or
pt
io
n E
m
ission
λabs = 593 nm (PBS buffer, pH 7.4)
λem
Data Sheet - SAB4600160
Figure 1).
Figure 1.
Absorption/Emission Spectra of CF640R Conjugated
Antibodies
525 575 625 675 725 775
Wavelength (nm)
A
bs
or
pt
io
n E
m
ission
λabs = 642 nm (PBS buffer, pH 7.4
Data Sheet - SAB4600162
Figure 1).
Figure 1.
Absorption/Emission Spectra of CF640R Conjugated
Antibodies
525 575 625 675 725 775
Wavelength (nm)
A
bs
or
pt
io
n E
m
ission
λabs = 642 nm (PBS buffer, pH 7.4
Data Sheet - SAB4600096
Figure 1).
Figure 1.
Absorption/Emission Spectra of CF594 Conjugated
Antibodies
475 525 575 625 675 725
Wavelength (nm)
A
bs
or
pt
io
n E
m
ission
λabs = 593 nm (PBS buffer, pH 7.4)
λem
Data Sheet - SAB4600164
Figure 1).
Figure 1.
Absorption/Emission Spectra of CF640R Conjugated
Antibodies
525 575 625 675 725 775
Wavelength (nm)
A
bs
or
pt
io
n E
m
ission
λabs = 642 nm (PBS buffer, pH 7.4
Data Sheet - SAB4600166
Figure 1).
Figure 1.
Absorption/Emission Spectra of CF640R Conjugated
Antibodies
525 575 625 675 725 775
Wavelength (nm)
A
bs
or
pt
io
n E
m
ission
λabs = 642 nm (PBS buffer, pH 7.4
Data Sheet - SAB4600098
Figure 1).
Figure 1.
Absorption/Emission Spectra of CF594 Conjugated
Antibodies
475 525 575 625 675 725
Wavelength (nm)
A
bs
or
pt
io
n E
m
ission
λabs = 593 nm (PBS buffer, pH 7.4)
λem
Data Sheet - SAB4600163
Figure 1).
Figure 1.
Absorption/Emission Spectra of CF640R Conjugated
Antibodies
525 575 625 675 725 775
Wavelength (nm)
A
bs
or
pt
io
n E
m
ission
λabs = 642 nm (PBS buffer, pH 7.4
Data Sheet - SAB4600094
Figure 1).
Figure 1.
Absorption/Emission Spectra of CF594 Conjugated
Antibodies
475 525 575 625 675 725
Wavelength (nm)
A
bs
or
pt
io
n E
m
ission
λabs = 593 nm (PBS buffer, pH 7.4)
λem
Data Sheet - SAB4600153
Figure 1).
Figure 1.
Absorption/Emission Spectra of CF640R Conjugated
Antibodies
525 575 625 675 725 775
Wavelength (nm)
A
bs
or
pt
io
n E
m
ission
λabs = 642 nm (PBS buffer, pH 7.4
Data Sheet - SAB4600103
Figure 1).
Figure 1.
Absorption/Emission Spectra of CF594 Conjugated
Antibodies
475 525 575 625 675 725
Wavelength (nm)
A
bs
or
pt
io
n E
m
ission
λabs = 593 nm (PBS buffer, pH 7.4)
λem
Data Sheet - SAB4600158
Figure 1).
Figure 1.
Absorption/Emission Spectra of CF640R Conjugated
Antibodies
525 575 625 675 725 775
Wavelength (nm)
A
bs
or
pt
io
n E
m
ission
λabs = 642 nm (PBS buffer, pH 7.4
Data Sheet - SAB4600104
Figure 1).
Figure 1.
Absorption/Emission Spectra of CF594 Conjugated
Antibodies
475 525 575 625 675 725
Wavelength (nm)
A
bs
or
pt
io
n E
m
ission
λabs = 593 nm (PBS buffer, pH 7.4)
λem
Data Sheet - SAB4600101
Figure 1).
Figure 1.
Absorption/Emission Spectra of CF594 Conjugated
Antibodies
475 525 575 625 675 725
Wavelength (nm)
A
bs
or
pt
io
n E
m
ission
λabs = 593 nm (PBS buffer, pH 7.4)
λem
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