126593

Albumin, Bovine Serum, Fraction V, RIA and ELISA Grade

• Sinonim: Albumin, Bovine Serum, Fraction V, RIA and ELISA Grade
• CAS Number: 9048-46-8
• MDL number: MFCD00011344
• NACRES: NA.25
• UNSPSC Code: 12352202
• Assay: ≥98% (agarose gel electrophoresis)
• Form: solid
• Impurities: ≤3.0 ash, ≤5.0% moisture

Properti

• description: Merck USA index - 14, 8468
• Quality Segment: 200
• assay: ≥98% (agarose gel electrophoresis)
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• impurities: ≤3.0 ash, ≤5.0% moisture
• color: off-white
• solubility: 0.15 M sodium chloride: 10 mg/mL
• cation traces: heavy metals: ≤10 ppm
• shipped in: ambient
• storage temp.: 2-8°C

Deskripsi

General description

Bovine serum albumin (BSA) is an α-helical, non-glycosylated globular protein with 17-disulfide bonds.

Albumin, Bovine Serum, Fraction V, RIA and ELISA Grade is a purified plasma protein that is useful as a stabilizing agent and as a carrier protein. It is prepared under non-denaturing conditions by modification of the Cohn procedure and caprylic acid heat-shock treatment.

Application

Albumin, Bovine Serum, Fraction V, RIA and ELISA Grade has been used:

• to block tissue sections of placenta in immunohistochemical (IHC) analysis
• as a component of Krebs buffer for in vitro glucose-stimulated insulin secretion (GSIS) experiments and drug/palmitate preparation
• as a vehicle control to incubate whole mouse blood to study plate aggregation in vivo

Biochem/physiol Actions

Bovine Serum Albumin (BSA), an important component in the cell culture media can transport fatty acids, hormones, and drugs. It is generally used as a blocking agent in enzyme-linked immunosorbent assay (ELISA).

Preparation Note

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Informasi Keselamatan

• Kelas Penyimpanan: 11 - Combustible Solids
• Ada Apa Sih?: WGK 3
• Titik Nyala (°F): Not applicable
• Titik Nyala (°C): Not applicable