1.01865

Readybag^® Half Fraser Broth with Supplements

acc. ISO 11290, 62 g, irradiated, For the primary selective enrichment of Listeria sp.

• Synonym(s): Demi Fraser broth, Fraser broth primary enrichment, Fraser Broth, Fraser Listeria selective enrichment Broth

Properties

• Agency: APHA; ISO 11133; ISO 11290
• Quality Level: 200
• form: medium granules (dehydrated (DCM))
• manufacturer/tradename: ReadyBag^®
• technique(s): microbiological culture: suitable
• pH: 7.0-7.4 (25 °C, 55 g/L in H₂O)
• solubility: 55 g/L
• application(s): food and beverages; microbiology; pathogen testing
• storage temp.: 15-25°C
• suitability: Listeria spp.

Description

General description

Half FRASER (Demi FRASER) broth has high nutrient content and the large buffer capacity that creates optimum growth conditions for Listeria. Lithium chloride, nalidixic acid, and acriflavine hydrochloride largely inhibit the growth of accompanying bacteria. Esculin and ammonium iron (III) citrate enables the detection of the β-D-glucosidase activity by the formation of a black complex of esculetin with iron (III) ions. However, this reaction is not exclusive to Listeria sp. and hence every primary and secondary enrichment in FRASER broth has to be sub-cultured on selective plating media.

Application

Half FRASER (Demi FRASER) broth with supplements is used for the primary selective enrichment of Listeria sp. from food and animal feed as well as from environmental samples and other materials.

Legal Information

READYBAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable