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1.07228

Millipore

GranuCult® Buffered Peptone Water

ISO 6579-1, ISO 19250, ISO 6887, ISO 21528-1, ISO 22964, FDA (BAM), APHA, EP, For the preliminary non selective enrichment of bacteria

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Synonym(s):
BPW, Buffered Peptone Solution, Peptone Water (buffered), Peptone Water, buffered, Peptone Water, phosphate-buffered, Tryptone Phosphate Water

Agency

APHA
EP
ISO 19250
ISO 21528-1
ISO 22964
ISO 6579-1
ISO 6887

Quality Level

reg. compliance

FDA (BAM)

form

medium granules (dehydrated (DCM))

manufacturer/tradename

GranuCult® prime

technique(s)

microbiological culture: suitable

pH

7.2 (37 °C, 25 g/L in H2O, after autoclaving)

solubility

25.5 g/L

bulk density

800 kg/m3

application(s)

food and beverages
microbiology
pathogen testing

storage temp.

15-25°C

suitability

Cronobacter spp.
Salmonella spp.
enterobacteriaceae

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EcoCult® Buffered Peptone Water ISO 6579, ISO 6887, ISO 21528, ISO 22964, FDA (BAM), APHA, EP 2.6.31, suitable for microbiology

1.40141

EcoCult® Buffered Peptone Water

Buffered Peptone Water according to ISO 6579, ISO 21528, ISO 22964, FDA-BAM, EP, sterile; γ-irradiated, ReadyBag®

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Buffered Peptone Water

Readybag® Buffered Peptone Water According to ISO 6579, ISO 21528, ISO 22964, FDA-BAM, APHA, and EP; For microbiology

1.00908

Readybag® Buffered Peptone Water

form

medium granules (dehydrated (DCM))

form

powder

form

medium granules

form

medium granules (dehydrated (DCM))

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

agency

APHA, ISO 19250, ISO 22964, ISO 6887, EP, ISO 6579-1, ISO 21528-1

agency

APHA, EP 2.6.31, ISO 21528, ISO 22964, ISO 6579, ISO 6887

agency

APHA, EP, FDA-BAM, ISO 21528, ISO 22964, according to ISO 6579

agency

APHA, EP, ISO 21528, ISO 22964, ISO 6579

application(s)

food and beverages
microbiology
pathogen testing

application(s)

food and beverages
pathogen testing
pharmaceutical, microbiology

application(s)

food and beverages
industrial qc
microbiology
pathogen testing
pharmaceutical

application(s)

food and beverages
microbiology
pathogen testing

storage temp.

15-25°C

storage temp.

10-30°C

storage temp.

15-25°C

storage temp.

15-25°C

General description

Buffered Peptone Water is rich in nutrients and produces high resuscitation rates for sub lethally injured bacteria and intense growth. The phosphate buffer system prevents bacterial damage caused by changes in the pH of the medium. Peptone including enzymatic digest of casein acts as a source of carbon, nitrogen, vitamins and minerals whilst sodium chloride maintains the osmotic balance.
GranuCult® is our new brand name for our existing granulated dehydrated culture media which apply to new EN ISO 11133:2014 and various other ISO standards and FDA-BAM / USDA-FSIS methods for testing of food and water samples. All these media are currently being updated to be fully compliant to the latest version of these standards and regulations with respect to composition and quality control and will be available shortly.

Application

Buffered Peptone Water is used in the testing of food and water samples. This product is suitable for the preliminary non selective enrichment of bacteria, particularly pathogenic Enterobacteriaceae such as Salmonella and Cronobacter, from food, animal feed, water, and other materials. The product is also suitable as a diluent for general use.

Linkage

Replaces: 1072285007; 1.07228

Analysis Note

Appearance (clearness): clear
Appearance (colour): yellowish
pH-value (25 °C): 6.8 - 7.2
Growth promotion test in accordance with the current version of DIN EN ISO 11133 and the harmonised method of EP, USP and JP.
Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013)): ≤ 100
Inoculum on reference medium (Escherichia coli ATCC 8739 (WDCM 00012)): ≤ 100
Inoculum on reference medium (Salmonella typhimurium ATCC 14028 (WDCM 00031)): ≤ 100
Inoculum on reference medium (Salmonella enteritidis ATCC 13076 (WDCM 00030)): ≤ 100
Inoculum on reference medium (Salmonella Abony NCTC 6017): ≤ 100
Growth (Escherichia coli ATCC 25922 (WDCM 00013)): good to very good
Growth (Escherichia coli ATCC 8739 (WDCM 00012)): good to very good
Growth (Salmonella typhimurium ATCC 14028 (WDCM 00031)): good to very good
Growth (Salmonella enteritidis ATCC 13076 (WDCM 00030)): good to very good
Growth (Salmonella Abony NCTC 6017): good to very good
Incubation:
18 ± 2 hours at 37 ± 1 °C
S.abony 18 - 24 hours at 30 - 35 °C

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3


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Articles

Shigella spp. are predominantly connected to humans and are rarely isolated from other animals, with the exception of primates.

Salmonella pass through the entire food chain from animal feed, primary production, and into households or food-service establishments.

Protocols

The new EN ISO 22964:2017 standard specifies a horizontal method for the detection of Cronobacter spp. in food, animal feed and environmental samples.

The International Organization for Standardization (ISO) published the revised EN ISO 21528-1 and EN ISO 21528-2 standards in 2017.

EN ISO 6579-1 standard specifies detection of Salmonella spp. in human consumption, animal feed, and environmental samples.

Listeria is a public health concern, affecting elderly, pregnant, newborns, and immunocompromised individuals.

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