Merck
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1.46476

Millipore

ReadyTube® Half Fraser Broth

For enrichment of samples for Listeria sp. and Listeria monocytogenes in accordance to ISO 11190, pkg of (225 mL in 250 mL bottle with blue screw cap and 3 loci (6 bottles per box))

Synonym(s):
Fraser Broth

Agency

ISO 11190

Quality Level

form

liquid (ready-to-use)

feature

ready-to-use

manufacturer/tradename

ReadyTube®

packaging

pkg of (225 mL in 250 mL bottle with blue screw cap and 3 loci (6 bottles per box))

pH

7.2 ( in H2O)

application(s)

environmental
food and beverages
microbiology

storage temp.

2-8°C

suitability

selective for Listeria spp.

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This Item
1.024491.018650120216
agency

ISO 11190

agency

APHA, ISO 11290

agency

APHA, ISO 11133, ISO 11290

agency

ISO 11290, according to ISO 11290

form

liquid (ready-to-use)

form

medium granules (dehydrated (DCM))

form

medium granules (dehydrated (DCM))

form

liquid

feature

ready-to-use

feature

-

feature

-

feature

ready-to-use

manufacturer/tradename

ReadyTube®

manufacturer/tradename

ReadyBag®

manufacturer/tradename

ReadyBag®

manufacturer/tradename

-

packaging

pkg of (225 mL in 250 mL bottle with blue screw cap and 3 loci (6 bottles per box))

packaging

pkg of 60 pieces, pkg of (Each sachet contains 12.5 g of granulated Half Fraser broth to which 225 mL of sterile water is added)

packaging

-

packaging

-

pH

7.2 ( in H2O)

pH

7.0-7.4 (25 °C, 55 g/L in H2O)

pH

7.0-7.4 (25 °C, 55 g/L in H2O)

pH

-

General description

ReadyTube® is ready to use media to provide the highest level of quality and testing confidence for improving accuracy and significantly reducing the risk of false results. ReadyTube® Half Fraser broth is a selective enrichment medium designed to help recovery of Listeria sp.

Application

ReadyTube® Fraser broth is used for the primary and secondary selective enrichment of Listeria sp. from food and animal feed as well as from environmental samples and other materials.

Analysis Note

This medium contains peptone, casein, yeast extract, and meat extract, which provide essential nutrients like carbon and nitrogenous compounds, including vitamins, amino acids, and trace ingredients. Phosphates buffer the medium while sodium chloride maintains osmotic equilibrium. Nalidixic acid and acriflavine inhibit the growth of Gram-negative and Gram-positive organisms respectively, except Listeria sp. Listeria sp. hydrolyze esculin to glucose and esculetin. The latter combines with ferric ions of ferric ammonium citrate, resulting in the formation of 6-7-Dihydroxycoumarin, a black-brown complex. Ferric ammonium citrate also enhances the growth of L. monocytogenes. The high salt tolerance (of sodium chloride) of Listeria is used as means to inhibit the growth of Enterococci. Lithium chloride is also used to inhibit Enterococci, which also possess the ability to hydrolyze esculin.

Legal Information

READYTUBE is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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