1.46719

Rambach Agar

Ready-to-Use Chromogenic Culture Medium for the Isolation of Salmonella sp. in 90mm plates

• Synonym(s): Chromogenic Salmonella Agar, Salmonella Isolation Agar acc RAMBACH

Properties

• Agency: ISO 6579
• Quality Level: 300
• form: (Solid in plate)
• shelf life: 4 mo.
• feature: ready-to-use
• packaging: pkg of (90 mm plate (20 or 100 plates per pack))
• pH: 7.3 ( in H₂O)
• application(s): food and beverages; industrial qc; microbiology
• storage temp.: 2-8°C
• suitability: Salmonella spp.

Description

General description

Rambach Agar plates are standard 90mm stackable specially designed plates that help increase safety during handling, transport, and incubation. Rambach agar is a selective media for isolation and differentiation of Salmonella sp. from Proteus sp. and other enteric bacteria. Production of acid from propylene glycol is a novel characteristic of Salmonella sp. and is utilized in these media.

Application

Rambach agar is a selective media for isolation and differentiation of Salmonella sp. from food and animal feed, water, and other materials.

Analysis Note

The nutritive substrates in the RAMBACH Agar enable Enterobacteriaceae to multiply readily. Sodium desoxycholate inhibits the accompanying Gram-positive flora. RAMBACH Agar enables species of Salmonella to be differentiated unambiguously from other bacteria by means of a new procedure, for which a patent application has been submitted. This is made possible by adding propylene glycol to the culture medium. Salmonellae form acid with propylene glycol, so that, in combination with a pH indicator, the colonies have a characteristic red colour. In order to differentiate coliforms from Salmonellae, the medium contains a chromogene indicating the presence of β-galactosidase splitting, a characteristic for coliforms. Coliform microorganisms grow as blue-green or blueviolet colonies. Other Enterobacteriaceae and Gram-negative
bacteria, such as Proteus, Pseudomonas, Shigella, S. typhi and S. parathyphi A grow as colourless-yellow colonies.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 2