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AB3790

Sigma-Aldrich

Anti-T7 Epitope Tag Antibody, T7 (MASMTGGQQMG)

Chemicon®, from rabbit

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eCl@ss:
32160702
NACRES:
NA.43

biological source

rabbit

Quality Level

antibody form

affinity purified immunoglobulin

clone

polyclonal

purified by

affinity chromatography

species reactivity (predicted by homology)

all

manufacturer/tradename

Chemicon®

technique(s)

immunocytochemistry: suitable
immunofluorescence: suitable
immunoprecipitation (IP): suitable
western blot: suitable

shipped in

wet ice

target post-translational modification

unmodified

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antibody form

affinity purified immunoglobulin

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affinity purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

clone

polyclonal

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polyclonal

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23c, monoclonal

clone

23c, monoclonal

manufacturer/tradename

Chemicon®

manufacturer/tradename

Chemicon®

manufacturer/tradename

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manufacturer/tradename

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technique(s)

immunocytochemistry: suitable, immunofluorescence: suitable, immunoprecipitation (IP): suitable, western blot: suitable

technique(s)

immunocytochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable

technique(s)

immunocytochemistry: suitable, immunofluorescence: suitable, immunoprecipitation (IP): suitable, western blot: suitable

technique(s)

immunocytochemistry: suitable, immunofluorescence: suitable, immunoprecipitation (IP): suitable, western blot: suitable

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

Specificity

T7 (MASMTGGQQMG). Rabbits were immunized with T7 (MASMTGGQQMG) conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support.

Immunogen

Epitope: T7 (MASMTGGQQMG)
MASMTGGQQMG (T7) conjugated to KLH

Application

Anti-T7 Epitope Tag Antibody, T7 (MASMTGGQQMG) detects level of T7 Epitope Tag & has been published & validated for use in IP, WB & IC.
Research Category
Epitope Tags & General Use
Research Sub Category
Epitope Tags
Western blot: 1:1,000-1:10,000 colorimetric; 1:1,000-1:30,000 ECL.

Immunocytochemistry: 1:100-1:400

ELISA: 1:100-1:500 for coating; 1:1,000-1:30,000 for primary.Immunofluorescence-suitable

Immunoprecipitation: 2-5μg/mL in 300-500μL of cell lysate (150-300μg/mL total protein). Lysis buffer 0.5% triton X-100 with proteinase inhibitors. Use high quality protein A beads or anti-rabbit conjugated beads for best results. Run bead-only (no T7 antibody) controls to mark bands related to protein A breakdown products.





Optimal working dilutions must be determined by the end user.

Physical form

Affinity purified immunoglobulin. Liquid in PBS, pH 7.2 with 0.1% sodium azide.

Storage and Stability

Maintain at 2-8°C in undiluted for up to 6 months from date of receipt.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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NF-κB is a key transcription factor that dictates the outcome of diverse immune responses. How NF-κB is regulated by multiple activating receptors that are engaged during natural killer (NK)-target cell contact remains undefined. Here we show that sole engagement of
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