All Photos(4)




Anti-GPC3 Antibody, clone 9C2

ascites fluid, clone 9C2, from mouse

Sign Into View Organizational & Contract Pricing

Select a Size

SGB, DGSX, MXR7, SDYS, SGBS, OCI-5, SGBS1, GTR2-2, Glypican-3, Intestinal protein OCI-5

biological source


Quality Level

antibody form

ascites fluid

antibody product type

primary antibodies


9C2, monoclonal

species reactivity

mouse, human


flow cytometry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable



UniProt accession no.

shipped in

wet ice

target post-translational modification


Gene Information

human ... GPC3(2719)

Related Categories

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
Anti-GPC3 IgG fraction of antiserum




antibody form

ascites fluid

antibody form

IgG fraction of antiserum

antibody form

purified immunoglobulin

antibody form

ascites fluid


9C2, monoclonal




1C9, monoclonal


4G10, monoclonal

species reactivity

mouse, human

species reactivity

mouse, horse, dog, rabbit, bovine, guinea pig, rat, human

species reactivity


species reactivity



flow cytometry: suitable, immunofluorescence: suitable, immunohistochemistry: suitable, western blot: suitable


immunoblotting: suitable, immunohistochemistry: suitable


immunoprecipitation (IP): suitable, indirect ELISA: suitable


immunohistochemistry: suitable, western blot: suitable









General description

Glypican-3 or GTR2-2 is the protein product of the gene GPC3. It is a cell surface heparin sulfate containing glycoprotein that plays an important role in the modulation of growth and development of mesodermal tissues and organs, as well as various cancers. Among its many known functions, it is a regulator of the dipeptidyl peptidase DPP4 (CD26). DPP4 plays an important role in glucose metabolism via its action on incretin and GLP-1 hormones. DPP4 also is a cancer marker and thought to function as a cancer suppressor. Thus acting as a regulator of DPP4, Glypican-3 (GPC3) participates in everything from glucose and blood sugar regulation to tumor growth. Glypican-3 (GPC3) is most highly expressed in the lung, liver and kidney, and typically a secreted form, secreted glypican-3, is found in the extracellular space generated as a cleavage product by the action of a convertase. Defects in GPC3 cause Simpson-Golabi-Behmel syndrome type 1 (SGBS1) or Simpson dysmorphia syndrome (SDYS). This is a condition characterized by pre-and postnatal overgrowth (gigantism) with visceral and skeletal anomalies. EMD-Millipore’s anti-GPC3 mouse monoclonal antibody, clone 9C2, has been tested in western blot against recombinant human protein, human HepG2, HEK293, Jurkat, and SK-N-SH cell line lysates, as well as rat PC-12 and mouse liver cell lysates. In addition, the clone as been successfully used in paraffin-embedded immunohistochemistry on human liver and breast cancer tissues and in immunofluorescent immunocytochemistry on human HeLa cells in culture. Finally, the clone has been tested successfully on human cells by flow cytometry and against the recombinant antigen by ELISA.


Purified recombinant fragment of human GPC3 expressed in E. Coli.


Detect Glypican using this mouse monoclonal antibody, Anti-GPC3 Antibody, clone 9C2 validated for use in western blotting, Immunofluorescence, IHC & Flow Cytometry.
Immunohistochemistry Analysis: A 1:100 dilution from a representative lot detected GPC3 in human liver and kidney tissues.

Immunofluorescence Analysis: A 1:200-1,000 dilution from a representative lot detected GPC3 in HeLa cells.

Flow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected GPC3 in Jurkat cells.

Optimal working dilutions must be determined by end user.
Research Category
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional


Evaluated by Western Blotting in mouse liver, HepG2, HEK293, Jurkat, SK-N-SH, PC-12, and F9 cell lysates.

Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected GPC3 in mouse liver, HepG2, HEK293, Jurkat, SK-N-SH, PC-12, and F9 cell lysates.

Target description

~66 kDa observed. Uncharacterized bands may appear in some lysate(s).

Physical form

Protein G Purified
Purified mouse monoclonal in PBS containing up to 0.1% sodium azide

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Mouse liver, HepG2, HEK293, Jurkat, SK-N-SH, PC-12, and F9 cell lysates


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


Health hazard

Signal Word


Hazard Statements

Hazard Classifications

Aquatic Chronic 3 - Repr. 1A

Storage Class Code

6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects



Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).


Product Number
Pack Size/Quantity

Additional examples:





enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Shiwei Han et al.
Oncotarget, 7(28), 44406-44416 (2016-06-04)
Gastric cancer is a prevalent tumor that is usually detected at an advanced metastatic stage. Currently, standard therapies are mostly ineffective. Here, we report that Glypican-3 (GPC3) is absent in invasive tumors and metastatic lymph nodes, in particular in aggressive
Mark S Cembrowski et al.
eLife, 7 (2018-10-31)
In the hippocampus, the classical pyramidal cell type of the subiculum acts as a primary output, conveying hippocampal signals to a diverse suite of downstream regions. Accumulating evidence suggests that the subiculum pyramidal cell population may actually be comprised of

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service