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MABC667

Sigma-Aldrich

Anti-GPC3 Antibody, clone 9C2

ascites fluid, clone 9C2, from mouse

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Synonym(s):
SGB, DGSX, MXR7, SDYS, SGBS, OCI-5, SGBS1, GTR2-2, Glypican-3, Intestinal protein OCI-5
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

ascites fluid

antibody product type

primary antibodies

clone

9C2, monoclonal

species reactivity

mouse, human

technique(s)

flow cytometry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable

isotype

IgG1

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... GPC3(2719)

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This Item
SAB2108511WH0002262M1MABC640
Anti-GPC3 IgG fraction of antiserum

Sigma-Aldrich

SAB2108511

Anti-GPC3

antibody form

ascites fluid

antibody form

IgG fraction of antiserum

antibody form

purified immunoglobulin

antibody form

ascites fluid

clone

9C2, monoclonal

clone

polyclonal

clone

1C9, monoclonal

clone

4G10, monoclonal

species reactivity

mouse, human

species reactivity

mouse, horse, dog, rabbit, bovine, guinea pig, rat, human

species reactivity

human

species reactivity

human

technique(s)

flow cytometry: suitable, immunofluorescence: suitable, immunohistochemistry: suitable, western blot: suitable

technique(s)

immunoblotting: suitable, immunohistochemistry: suitable

technique(s)

immunoprecipitation (IP): suitable, indirect ELISA: suitable

technique(s)

immunohistochemistry: suitable, western blot: suitable

isotype

IgG1

isotype

-

isotype

IgG2aκ

isotype

IgG1

General description

Glypican-3 or GTR2-2 is the protein product of the gene GPC3. It is a cell surface heparin sulfate containing glycoprotein that plays an important role in the modulation of growth and development of mesodermal tissues and organs, as well as various cancers. Among its many known functions, it is a regulator of the dipeptidyl peptidase DPP4 (CD26). DPP4 plays an important role in glucose metabolism via its action on incretin and GLP-1 hormones. DPP4 also is a cancer marker and thought to function as a cancer suppressor. Thus acting as a regulator of DPP4, Glypican-3 (GPC3) participates in everything from glucose and blood sugar regulation to tumor growth. Glypican-3 (GPC3) is most highly expressed in the lung, liver and kidney, and typically a secreted form, secreted glypican-3, is found in the extracellular space generated as a cleavage product by the action of a convertase. Defects in GPC3 cause Simpson-Golabi-Behmel syndrome type 1 (SGBS1) or Simpson dysmorphia syndrome (SDYS). This is a condition characterized by pre-and postnatal overgrowth (gigantism) with visceral and skeletal anomalies. EMD-Millipore’s anti-GPC3 mouse monoclonal antibody, clone 9C2, has been tested in western blot against recombinant human protein, human HepG2, HEK293, Jurkat, and SK-N-SH cell line lysates, as well as rat PC-12 and mouse liver cell lysates. In addition, the clone as been successfully used in paraffin-embedded immunohistochemistry on human liver and breast cancer tissues and in immunofluorescent immunocytochemistry on human HeLa cells in culture. Finally, the clone has been tested successfully on human cells by flow cytometry and against the recombinant antigen by ELISA.

Immunogen

Purified recombinant fragment of human GPC3 expressed in E. Coli.

Application

Detect Glypican using this mouse monoclonal antibody, Anti-GPC3 Antibody, clone 9C2 validated for use in western blotting, Immunofluorescence, IHC & Flow Cytometry.
Immunohistochemistry Analysis: A 1:100 dilution from a representative lot detected GPC3 in human liver and kidney tissues.

Immunofluorescence Analysis: A 1:200-1,000 dilution from a representative lot detected GPC3 in HeLa cells.

Flow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected GPC3 in Jurkat cells.

Optimal working dilutions must be determined by end user.
Research Category
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional

Quality

Evaluated by Western Blotting in mouse liver, HepG2, HEK293, Jurkat, SK-N-SH, PC-12, and F9 cell lysates.

Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected GPC3 in mouse liver, HepG2, HEK293, Jurkat, SK-N-SH, PC-12, and F9 cell lysates.

Target description

~66 kDa observed. Uncharacterized bands may appear in some lysate(s).

Physical form

Protein G Purified
Purified mouse monoclonal in PBS containing up to 0.1% sodium azide

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Mouse liver, HepG2, HEK293, Jurkat, SK-N-SH, PC-12, and F9 cell lysates

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Aquatic Chronic 3 - Repr. 1A

Storage Class Code

6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Shiwei Han et al.
Oncotarget, 7(28), 44406-44416 (2016-06-04)
Gastric cancer is a prevalent tumor that is usually detected at an advanced metastatic stage. Currently, standard therapies are mostly ineffective. Here, we report that Glypican-3 (GPC3) is absent in invasive tumors and metastatic lymph nodes, in particular in aggressive
Mark S Cembrowski et al.
eLife, 7 (2018-10-31)
In the hippocampus, the classical pyramidal cell type of the subiculum acts as a primary output, conveying hippocampal signals to a diverse suite of downstream regions. Accumulating evidence suggests that the subiculum pyramidal cell population may actually be comprised of

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