Merck
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MHA000P2S

Millipore

m-Heterotrophic Plate Count Broth

2ml ampoule for the recovery of stressed heterotrophic bacteria found in various types of water. Also suitable for water samples with low counts.

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Synonym(s):
m-HPC Broth
eCl@ss:
32160801
NACRES:
NA.74

Quality Level

form

liquid

shelf life

12 mo.

feature

ready-to-use

packaging

pkg of 2 mL
pack of 50 tests

technique(s)

cell counting: suitable (total viable count (TVC))

color

clear tan to light amber

input

sample type cider
sample type beverage(s)
sample type cosmetics
sample type bottled water
sample type food(s)
sample type beer
sample type wine
sample type soft drink
sample type sport drink

pH

7.1±0.2 (25.0 °C)

application(s)

clinical
cosmetics
environmental
food and beverages
industrial qc
microbiology
water monitoring

compatibility

for use with MF-method
for use with Microfil®
for use with MicropreSure®
for use with Milliflex®

shipped in

wet ice

storage temp.

2-8°C

suitability

nonselective for

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This Item
MHA00P2TTMHA000P2EMHA000P2P
shelf life

12 mo.

shelf life

12 mo.

shelf life

12 mo.

shelf life

12 mo.

feature

ready-to-use

feature

ready-to-use

feature

ready-to-use

feature

ready-to-use

packaging

pkg of 2 mL, pack of 50 tests

packaging

ampule of 2 mL, pack of 50 tests

packaging

ampule of 2 mL, pack of 50 tests

packaging

pkg of 2 mL, pack of 50 tests

technique(s)

cell counting: suitable (total viable count (TVC))

technique(s)

cell counting: suitable (total viable count (TVC))

technique(s)

-

technique(s)

-

pH

7.1±0.2 (25.0 °C)

pH

7.0±0.2 (25.0 °C)

pH

7.2±0.2 (25.0 °C)

pH

7.1±0.2 (25.0 °C)

General description

Heterotrophs are organisms including bacteria, yeasts and moulds that require an external source of organic carbon for growth. The heterotrophic plate count (HPC), formerly known as the standard plate count, is a procedure for estimating the number of live heterotrophic bacteria in water.{14} This test can provide useful information about water quality and supporting data on the significance of coliform test results. Heterotrophic Plate Count Broth can be employed for the determination of bacteria by the membrane filter method. Peptic digest of animal tissue is the source of nutrients for organisms, which are not highly fastidious. Gelatin is utilized by microorganisms through a proteolytic mechanism. The addition of glycerol to the basal medium provides a source of carbon and energy

Application

m-HPC broth is used in the recovery of stressed heterotrophic bacteria found in various types of water. Also suitable for water samples with low counts. Beer, Bottled Water, Cider, Soft Drink, Sport Drink and Wine. The media finds its application in Clinical, Cosmetics, Environmental Analysis, Food and Beverage and Industrial QC.

Packaging

2 mL plastic ampoules

Specifications

Incubation Temperature: 30–35 °C
Incubation Time: 48–72 hrs
Standard Methods for the Examination of Water and Wastewater

Preparation Note

Prior to use, warm media at 30-50 °C until liquefied. Do not microwave.

Culture Medium

m-HPC

Analysis Note

Colonies appear clear to creamy white, some may produce pigment
QC organisms
E. coli (ATCC 25922)

S. epidermidis (ATCC 14990)
Target Microorganisms: Total Count

Legal Information

MICROFIL is a registered trademark of Merck KGaA, Darmstadt, Germany
MICROPRESURE is a registered trademark of Merck KGaA, Darmstadt, Germany
MILLIFLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Customers Also Viewed

Standard plate count methodology: a new membrane filter procedure for potable water and swimming pools
Taylor R and Geldreich EE
J. Amer. Water Works Assoc., 71 (1979)

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