Merck
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MSIPS4510

Millipore

Multiscreen® 96 well Plate, hydrophobic PVDF membrane

pore size 0.45 μm, sterile

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eCl@ss:
32039006
NACRES:
NB.22

material

PVDF membrane (hydrophobic)
acrylic
flat bottom wells

Quality Level

description

Sterile, clear 96-well filter plate with 0.45 um pore size Hydrophobic PVDF membrane for ELISPOT Assays. Comes in a pack of 10.

sterility

sterile

product line

MultiScreen®

feature

hydrophobic
lid

manufacturer/tradename

MultiScreen®

parameter

50-250 μL sample volume (per well)

technique(s)

ELISPOT: suitable

filtration area

0.28 cm2

plate size

96 wells

well maximum volume

300 μL

working volume

50-250 μL

matrix

Immobilon®-P

pore size

0.45 μm pore size

input

sample type hematopoietic stem cell(s)
sample type pancreatic stem cell(s)
sample type: mouse embryonic stem cell(s)
sample type epithelial cells
sample type induced pluripotent stem cell(s)
sample type mesenchymal stem cell(s)
sample type: human embryonic stem cell(s)
sample type neural stem cell(s)
sample type cardiac stem cell(s)

binding type

high binding surface

shipped in

ambient

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This Item
M8IPS4510MAIPS4510MAIPSWU10
sterility

sterile

sterility

non-sterile

sterility

sterile

sterility

sterile

feature

hydrophobic, lid

feature

hydrophobic, lid

feature

hydrophobic, lid

feature

hydrophobic, lid

manufacturer/tradename

MultiScreen®

manufacturer/tradename

MultiScreen®

manufacturer/tradename

MultiScreen®

manufacturer/tradename

MultiScreen®

well maximum volume

300 μL

well maximum volume

300 μL

well maximum volume

300 μL

well maximum volume

300 μL

working volume

50-250 μL

working volume

50-250 μL

working volume

50-250 μL

working volume

50-250 μL

General description

Filter Code: IP
No matter what application you’re working with, Millipore has a plate developed to meet your specific needs. MultiScreen plates have a long history as a reliable tool for the advancement of drug discovery and life science research.

HTS Filter Plates (Plate catalogue numbers that start with MS)
- MultiScreenHTS plates are specifically developed for high-throughput use with automated work stations. Rigid sidewalls improve handling and ample surfaces are provided for bar code labels. Wells are individually sealed to prevent incubation crosstalk and the underdrain is removable for access to the filters. Filter plates are available in 96- and 384-well platforms with a broad selection of membranes and plastics.

- Millipore’s new MultiScreen HTS+ Hi flow filter plate design lessens non-specific binding and reduces variability in both background and signal intensities specifically for biochemical screening assays.

Classic Filter Plates (Plate catalogue numbers that start with MA)
- The original multiwell design is valuable for many applications which require immersing the filter in an assay tray. The easily removable underdrain and unique plate design are ideal for assays such as Elispot, PAMPA, cell screening (migration, invasion or chemotaxis) or for topcount detection users.

Accessory – MultiScreenHTS Vacuum Manifold
- The MultiScreenHTS Vacuum manifold was designed specifically to work with MultiScreenHTS filter plates. The combination results in optimal filtration characteristics and seamless automation integration.

Millicell Assay Plates
- In addition to the traditional assay plate format, filter plates are also available with cell culture trays for growing and assaying suspension and adherent cell lines. Cells grown on filter plates differentiate better than those grown on traditional plastic substrates, resulting in more in vivo-like results This filter plate/tray combination can be used for a variety of cellular assays, including, transport, toxicity and screening assays.

Application

Research Category
Assays

Cell Culture
Sterile, clear 96-well filter plate with 0.45 um pore size Hydrophobic PVDF membrane for ELISPOT Assays. Comes in a pack of 10.

Physical form

Color Code: Clear

Other Notes

Automation Compatible: Yes

Legal Information

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany
MULTISCREEN is a registered trademark of Merck KGaA, Darmstadt, Germany

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Articles

A Highly Sensitive IFN-γ ELISpot Assay to Quantify Cellular Immune Responses to Previous Viral Infection

This study presents an example of a typical ELISpot assay for quantifying the number of pre-existing, antigen-reactive T cells from peripheral blood mononuclear cell (PBMC) samples obtained from donors previously infected with cytomegalovirus (CMV).

The ELISpot Assay Enables Functional Analysis of Cellular Immunology

The enzyme-linked immunosorbent spot (ELISpot ) assay enables visualization of multiple secretory products from a single responding cell. The ELISpot provides both qualitative (type of immune protein) and quantitative (number of responding cells) information.

Protocols

IFN-γ ELISpot Assays on MultiScreen® IP

The ELISpot (Enzyme Linked Immuno-Spot) assay provides an effective method of measuring the antibody or cytokine production of immune cells on the single cell level.

Guidelines for Bioassays on MultiScreen Filter Plates: Protein Binding and Cell Loading

Guidelines for various bioassays on the MultiScreen system, which makes it possible to carry out an assay from sample incubation to scintillation counting in a 96-well plate.

Related Content

Chemical Compatibility Guide for MultiScreen® Filter Plates

This provides information about the chemical compatibility of MultiScreen Filter Plates with different reagents.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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