11721615001

DNA Molecular Weight Marker XV (Expand^™ DNA Molecular Weight Marker)

pkg of 50 μg (100 μg/ml), solution

Properties

• form: solution
• packaging: pkg of 50 μg (100 μg/ml)
• manufacturer/tradename: Roche
• storage temp.: −20°C

Description

General description

Fragment mixture prepared by cleavage of λDNA with various restriction endonucleases.
Size Range: 2392 to 48502 bp

Application

DNA Molecular Weight Marker XV allows accurate sizing of DNA fragments generated by the Expand Long Template PCR System or restriction digest of high molecular weight DNA cleaved with rare-cutting restriction endonucleases and separated on agarose gels.
This marker may be used for accurate molecular weight determination of double-stranded DNA fragments generated by:

• Restriction digests
• PCR
• RT-PCR
• Pullse-field gel electrophoresis

Components

Ready-to-use solution, 100μg/mL, in TE buffer (10mM Tris-HCl, 1mM EDTA, pH 8.0).

Sequence

The mixture contains twenty-four fragments with the following base pair lengths (1 base pair = 660 daltons): 2392, 3574, 7601, 8113, 9688, 10086, 11205, 11848, 12379, 13282, 14183, 15258, 15262, 16710, 18780, 19944, 20323, 22010, 24918, 26718, 29027, 32745, 38412, and 48502 (undigested λDNA).
Note: Fragment lengths are derived from computer analysis of the DNA sequence. Depending on the size range of the marker, the smallest fragments will only be visible on overloaded gels.The DNA fragments 15262, 15258, 19944, and 20323 appear as a single band on a 0.4% agarose gel. The DNA fragments 9688, 13282, and 22010 bp appear brighter on a 0.4% agarose gel. The 48,502 bp "fragment" is undigested λDNA.

Unit Definition

50 μg = 1A₂₆₀ unit

Preparation Note

Working solution: Lysis buffer/wash buffer 1
The kit contains reagents for 125 ml of lysis buffer/wash buffer 1. Prepare at least a minimal volume of 25 ml, sufficient for four immunoprecipitations.
To prepare 25 ml of lysis buffer/wash buffer 1 mix 5 ml core buffer, 3.75 ml NaCl, 2.5 ml detergent mix and 1 cOmplete tablet. Add water to a final volume of 25 ml.
Solution is stable at 2 to 8 °C for 24 hours. When stored in aliquots at -15 to -25 °C, the solution is stable for at least four weeks. Mix thoroughly after thawing.

Wash buffer 2
The kit contains reagents for 50 ml of wash buffer 2. 2 ml of this buffer is required for one immunoprecipitation.
To prepare 50 ml of wash buffer 2 mix 10 ml core buffer, 25 ml NaCl and 0.5 ml detergent mix. Add water to a final volume of 50 ml.
Solution is stable at 2 to 8 °C. For longer periods, store aliquots at -15 to -25 °C. Mix thoroughly after thawing.

Wash buffer 3
The kit contains reagents for 25 ml of wash buffer 3. 1 ml of this buffer is required for one immunoprecipitation.
To prepare 25 ml mix 1 ml core buffer and 0.25 ml detergent mix. Add water to a final volume of 25 ml.
Solution is stable at 2 to 8 °C. For longer periods, store aliquots at -15 to -25 °C. Mix thoroughly after thawing.
Storage conditions (working solution): 2 to 8 °C
Once thawed we recommend further storage at 2 to 8 °C.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Legal Information

Expand is a trademark of Roche

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point(F): does not flash
• Flash Point(C): does not flash