Merck
All Photos(1)

11721933001

Roche

DNA Molecular Weight Marker XIV (100 bp ladder)

solution, pkg of 50 μg (in 200 μl)

All Photos(1)
Synonym(s):
DNA marker

form

solution

Quality Level

100

packaging

pkg of 50 μg (in 200 μl)

manufacturer/tradename

Roche

storage temp.

−20°C

Related Categories

Compare Similar Items

View Full Comparison

1 of 4

This Item
117219250011120926400111449460001
DNA Molecular Weight Marker XIV (100 bp ladder) solution, pkg of 50 μg (in 200 μl)

Roche

11721933001

DNA Molecular Weight Marker XIV (100 bp ladder)

DNA Molecular Weight Marker XIII (50 bp ladder) pkg of 50 μg (250 μg/ml), solution

Roche

11721925001

DNA Molecular Weight Marker XIII (50 bp ladder)

DNA Molecular Weight Marker VII pkg of 50 μg (in 200 μl), solution

Roche

11209264001

DNA Molecular Weight Marker VII

DNA Molecular Weight Marker IX solution, pkg of 50 μg (in 200 μl)

Roche

11449460001

DNA Molecular Weight Marker IX

form

solution

form

solution

form

solution

form

solution

packaging

pkg of 50 μg (in 200 μl)

packaging

pkg of 50 μg (250 μg/ml)

packaging

pkg of 50 μg (in 200 μl)

packaging

pkg of 50 μg (in 200 μl)

manufacturer/tradename

Roche

manufacturer/tradename

Roche

manufacturer/tradename

Roche

manufacturer/tradename

Roche

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

General description

Fragment mixture prepared by cleavage of a specially constructed plasmid with restriction endonucleases.
Size Range: 100 to 1500 bp and an additional band of 2642 bp

Application

DNA Molecular Weight Marker XIV (100 bp ladder) allows accurate sizing of DNA fragments generated by PCR or restriction digest or RT-PCR, following separation on agarose gels. The DNA molecular weight marker can be used in conjunction with DNA Molecular Weight Marker XIII (50 bp ladder) for precise size determination.
Electrophoretic separation of this molecular weight marker results in a regular pattern. The fragments have 5′-protruding ends and can be labeled with radioactive nucleotides (e.g.,32P-dTTP or 32P-dGTP) by standard filling-in reactions. End-labeling reactions can be performed with a radioactive or nonradioactive dideoxynucleotide (e.g., DIG-11-ddUTP) and Terminal Transferase.
DNA Molecular Weight Marker XIV has been used in gel electrophoresis.

Sequence

The mixture contains 15 double-stranded DNA fragments with the following base pair lengths (1 base pair = 660 daltons): 100, 200, 300, 400, 500, 600, 700, 800, 900, 1000, 1100, 1200, 1300, 1400, 1500, and an additional band of 2642 bp. The 500-bp and 1000-bp banding pattern is two to three times brighter.
Note: Fragment lengths are derived from computer analysis of the DNA sequence. Depending on the size range of the marker, the smallest fragments will only be visible on overloaded gels.To make them easy to identify, the 500 and 1,000 bp bands are two to three times as bright as the other bands in the ladder.

Unit Definition

50 μg = 1A260 unit

Physical form

Ready-to-use solution, 250 μg/ml, in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 8.0).

Other Notes

For life science research only. Not for use in diagnostic procedures.

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

does not flash

Flash Point(C)

does not flash

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (Example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service