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Water, PCR Grade

liquid, pkg of 25 mL (03315959001 [1 x 25 ml]), pkg of 25 mL (03315932001 [25 x 1 ml]), pkg of 100 mL (03315843001 [4 x 25 ml])

EC Number:



Quality Level


pkg of 100 mL (03315843001 [4 x 25 ml])
pkg of 25 mL (03315932001 [25 x 1 ml])
pkg of 25 mL (03315959001 [1 x 25 ml])



storage temp.


General description

Water, PCR Grade is specially purified, double-distilled, deionized, and autoclaved. The production process ensures that even according to Roche ′s strict Quality Control procedures the water does not contain detectable amounts of nucleic acid. It is routinely tested in PCR with conventional thermal cyclers and with LightCycler® Instruments.


This product is specially tested and qualified for use in all PCR or RT-PCR. It can be used in conjunction with all end-point or real-time PCR assays or whenever highest quality water is needed. All of the LightCycler® kits and some of the amplification products — available from Roche Applied Science — contain this Water, PCR Grade.


25, 100 mL


Function test: Each lot of Water, PCR Grade is tested in PCR (amplification of a 0.5 kb target from 0.01 ng λDNA). When the PCR product is analyzed by gel electrophoresis, a clear, defined band must be seen on the gel.
Absence of nucleases: The water is tested with various nucleic acids that could serve as nuclease substrates. No endonucleases, ribonucleases or nicking activity are detected according to the current Quality procedures.

Preparation Note

Storage conditions (working solution): Store a working solution for a short period of time (e.g. 1 week) at 2 to 8 °C.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Legal Information

LightCycler is a registered trademark of Roche

Storage Class Code

12 - Non Combustible Liquids



Flash Point(F)

does not flash

Flash Point(C)

does not flash

Certificate of Analysis

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Certificate of Origin

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Product Information Sheet

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