17173

Rappaport Vassiliadis Broth, modified

suitable for microbiology, NutriSelect^® Plus

• Synonym(s): Magnesium chloride Malachite Green Broth, Modified Rappaport Vassiliadis Broth, RV Broth, Salmonella Enrichment Broth acc. to Rappaport and Vassiliadis
• NACRES: NA.85

Properties

• Agency: according to ISO 19250:2010; according to ISO 6579-1:2017
• Quality Level: 200
• sterility: non-sterile
• form: powder
• shelf life: limited shelf life, expiry date on the label
• manufacturer/tradename: NutriSelect^® Plus
• technique(s): microbiological culture: suitable
• final pH: 5.2±0.2 (25 °C)
• application(s): environmental; food and beverages; food and beverages; pathogen testing; microbiology
• suitability: selective for Salmonella spp.

Description

Application

Used as a selective enrichment medium for the isolation of Salmonella species from food and environmental specimens.

Components

Papaic digest of soyabean meal 5 g/1110 mL;
Sodium chloride 8 g/1110 mL;
Monopotassium phosphate 1.6 g/1110 mL;
Magnesium chloride anhydrous 18.7 g/l (is equivalent to 40 g/1110 mL Magnesium chloride hexahydrate);
Malachite Green 0.04 g/1110 ml

Preparation Note

Suspend 30.0 g in 1 litre distilled water. Heat gently if necessary to dissolve the medium completely. Dispense as desired into tubes and sterilize by autoclaving at 115°C for 15 minutes.

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves