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Millipore

Listeria mono Differential Agar acc. Ottaviani & Agosti (Base)

NutriSelect® Plus, suitable for microbiology, according to ISO 11290-1:2017, according to ISO 11290-2:2017

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Synonym(s):
Agar Listeria Ottaviani & Agosti, Listeria monocytogenes Differential Agar (Base)
NACRES:
NA.85

Agency

according to ISO 11290-1:2017
according to ISO 11290-2:2017

Quality Level

sterility

non-sterile

form

powder

shelf life

limited shelf life, expiry date on the label

composition

agar, 15.0 g/L
casein enzymic hydrolysate, 6.0 g/L
chromogenic substrate, 0.05 g/L
disodium hydrogen phosphate anhydrous, 2.5 g/L
glucose, 2.0 g/L
lithium chloride, 10.0 g/L
magnesium glycerophosphate, 1.0 g/L
magnesium sulfate, 0.5 g/L
meat peptone, 18.0 g/L
sodium chloride, 5.0 g/L
sodium pyruvate, 2.0 g/L
yeast extract, 10.0 g/L

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

final pH

7.2±0.2 (25 °C)

application(s)

agriculture
environmental
food and beverages

microbiology

suitability

selective and differential for Listeria spp.

Application

L. mono differential Agar has been recommended by ISO Committee for the selective and differential isolation of Listeria monocytogenes.

Preparation Note

Suspend 36.0 g in 465 ml distilled water. Boil gently to dissolve the medium completely. Sterilize by autoclaving at 121°C for 15 min. Cool to 45-50°C. Aseptically add sterile contents of 1 vial of Listeria mono Enrichement Supplement I (Cat. No. 03708) and sterile rehydrated contents of Listeria mono Selective Supplement (Cat. No. 40784). Mix well and pour into sterile petri plates.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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