Merck
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85927

Millipore

ECC ChromoSelect Selective Agar

suitable for microbiology, NutriSelect® Plus

Synonym(s):
E. coli-Coliform Selective Agar ChromoSelect, ECC Selective Agar ChromoSelect
MDL number:
NACRES:
NA.85

sterility

non-sterile

Quality Level

form

powder

shelf life

limited shelf life, expiry date on the label

composition

agar, 10.0 g/L
casein enzymic hydrolysate, 3.3 g/L
chromogenic mixture, 0.43 g/L
disodium hydrogen phosphate, 1 g/L
peptone, special, 6.0 g/L
sodium chloride, 2 g/L
sodium dihydrogen phosphate, 0.6 g/L
sodium pyruvate, 1 g/L
sorbitol, 1 g/L
Tergitol
7, 0.15 g/L

tryptophan, 1 g/L

manufacturer/tradename

NutriSelect® Plus

final pH

6.8±0.2 (25 °C)

application(s)

agriculture
bioburden testing
environmental
food and beverages
water monitoring

microbiology

suitability

selective and differential for Citrobacter spp.
selective and differential for Enterobacter spp.
selective and differential for Escherichia coli
selective and differential for Salmonella spp.
selective and differential for Shigella spp.
selective and differential for coliforms
selective and differential for enterobacteriaceae

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This Item
819389232470722
Coliform ChromoSelect Agar suitable for microbiology, NutriSelect® Plus

Millipore

81938

Coliform ChromoSelect Agar

form

powder

form

powder

form

powder

form

powder

shelf life

limited shelf life, expiry date on the label

shelf life

limited shelf life, expiry date on the label

shelf life

limited shelf life, expiry date on the label

shelf life

limited shelf life, expiry date on the label

composition

agar, 10.0 g/L , casein enzymic hydrolysate, 3.3 g/L , chromogenic mixture, 0.43 g/L , disodium hydrogen phosphate, 1 g/L , peptone, special, 6.0 g/L , sodium chloride, 2 g/L , sodium dihydrogen phosphate, 0.6 g/L , sodium pyruvate, 1 g/L , sorbitol, 1 g/L , Tergitol
7, 0.15 g/L , tryptophan, 1 g/L

composition

agar, 12 g/L , chromogenic mixture, 0.2 g/L , dipotassium hydrogen phosphate, 3 g/L , peptone, special, 3 g/L , potassium dihydrogen phosphate, 1.7 g/L , sodium chloride, 5 g/L , sodium lauryl sulfate, 0.1 g/L , sodium pyruvate, 1 g/L , tryptophan, 1 g/L

composition

agar, 15.0 g/L , casein enzymic hydrolysate, 15.0 g/L , chromogenic mixture, 10.17 g/L , papaic digest of soyabean meal, 5.0 g/L , sodium chloride, 5.0 g/L , sodium deoxycholate, 0.5 g/L , sodium thiosulfate, 1.0 g/L

composition

agar, 15 g/L , bile salt mixture, 1.5 g/L , casein enzymic hydrolysate, 20 g/L , X-glucuronide, 0.075 g/L

manufacturer/tradename

NutriSelect® Plus

manufacturer/tradename

NutriSelect® Plus

manufacturer/tradename

NutriSelect® Plus

manufacturer/tradename

NutriSelect® Plus

final pH

6.8±0.2 (25 °C)

final pH

6.8±0.2 (25 °C)

final pH

7.3±0.2 (25 °C)

final pH

7.2±0.2 (25 °C)

Application

ECC ChromoSelect Selective Agar is a selective medium recommended for the simultaneous detection of Escherichia coli and coliforms in water and food samples. The ingredients help even the sublethally injured coliforms to grow rapidly, Tergitol inhibits Gram-positive as well as some Gram-negative bacteria other than coliforms. The chromogenic mixture contains two chromogenic substrates as Salmon-GAL and X-glucuronide. The enzyme β-D-galactosidase produced by coliforms cleaves Salmon-GAL, resulting in the salmon-to-red coloration of coliform colonies. The enzyme β-D-glucuronidase produced by E. coli, cleaves X-glucuronide. E. coli forms dark blue-to-violet colored colonies due to cleavage of both Salmon-GAL and X-glucuronide. The addition of tryptophan improves the indole reaction. To confirm E. coli, add a drop of Kovac′s reagent (Catalog No. 60983) on the dark blue-to-violet colony. Formation of cherry-red color indicates the positive reaction.

Preparation Note

Suspend 26.5 g in 1 litre distilled water. Heat in a boiling water bath or in a free flowing steam, with stirring to dissolve the medium completely (approximately 35 minutes). Medium may show haziness, but it does not affect the performance. If desired to inhibit Pseudomonas and Aeromonas species add 5 mg Cefsulodin (Cat. No. 22126) for surface and pour plate method and 10 mg Cefsulodin for membrane filter technique. Mix well.

Reconstitution

Suspend 26.5 g in 1 liter of distilled water. Heat in a boiling water bath or in a free flowing steam, with stirring to dissolve the medium completely (approximately 35 minutes). Medium may show haziness, but it does not affect the performance. If desired to inhibit Pseudomonas and Aeromonas species, add 5 mg of cefsulodin for surface and pour plate method and 10 mg of cefsulodin for membrane filter technique. Mix well.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
TERGITOL is a trademark of The Dow Chemical Company ("Dow") or an affiliated company of Dow

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Skin Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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