Merck
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92322

Millipore

Rappaport Vassiliadis medium (base), modified, semi-solid

suitable for microbiology, NutriSelect® Plus

All Photos(2)
Synonym(s):
MSRV medium (base), Modified Semisolid Rappaport Vassiliadis Medium (base)
NACRES:
NA.85

sterility

non-sterile

Quality Level

200

form

powder

shelf life

limited shelf life, expiry date on the label

composition

agar, 2.7 g/L
casein peptone, 4.59 g/L
magnesium chloride, 10.93 g/L
malachite green, 0.037 g/L
mono-potassium phosphate, 1.47 g/L
sodium chloride, 7.34 g/L
tryptose, 4.59 g/L

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

final pH

5.2±0.2 (25 °C)

application(s)

environmental
food and beverages
sample preparation
microbiology

storage temp.

2-8°C

suitability

selective for Salmonella spp.

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1.098781.07700911461
Rappaport Vassiliadis medium (base), modified, semi-solid suitable for microbiology, NutriSelect® Plus

Millipore

92322

Rappaport Vassiliadis medium (base), modified, semi-solid

Modified Semi-solid RAPPAPORT-VASSILIADIS medium (base) pkg of 500 g, ISO 6579, for Salmonella spp.

Millipore

1.09878

Modified Semi-solid RAPPAPORT-VASSILIADIS medium (base)

GranuCult® RAPPAPORT-VASSILIADIS-Soya broth (base) ISO 6579, for Salmonella spp.

Millipore

1.07700

GranuCult® RAPPAPORT-VASSILIADIS-Soya broth (base)

Polyester bis-MPA dendron, 2 NHBoc, 1 NHS (core) generation 1

Sigma-Aldrich

911461

Polyester bis-MPA dendron, 2 NHBoc, 1 NHS (core)

sterility

non-sterile

sterility

-

sterility

-

sterility

-

form

powder

form

powder (dehydrated (DCM))

form

medium granules (dehydrated (DCM))

form

solid or liquid

shelf life

limited shelf life, expiry date on the label

shelf life

-

shelf life

-

shelf life

-

composition

agar, 2.7 g/L , casein peptone, 4.59 g/L , magnesium chloride, 10.93 g/L , malachite green, 0.037 g/L , mono-potassium phosphate, 1.47 g/L , sodium chloride, 7.34 g/L , tryptose, 4.59 g/L

composition

-

composition

-

composition

-

manufacturer/tradename

NutriSelect® Plus

manufacturer/tradename

GranuCult®

manufacturer/tradename

GranuCult®

manufacturer/tradename

-

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

-

Application

The modified Semi-Solid Rappaport Vassiliadis medium is formulated according De Smedt and et al. for enrichment of Salmonellae.

Preparation Note

Suspend 31.6 g in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. DO NOT AUTOCLAVE. Cool to 45-50°C and aseptically add 20 mg/L of Novobiocin (Cat. No. 74675). Mix well and pour into sterile petri plates. Keep plates in a fresh place to settle the gel (1 hour minimum) and handle with care because the medium is only semi-solid.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

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