D3937

DirectLoad^™ 1 kb DNA Ladder

ready-to-use marker for DNA electrophoresis

• Synonym(s): 1 kb marker, 1kb gel ladder, 1kb gel marker, 1kb ladder for gel electrophoresis, 1kb marker for gel electrophoresis, DNA ladder, DNA marker, DirectLoad^™ marker, RTU DNA ladder, RTU DNA marker, agarose gel electrophoresis ladder, agarose gel electrophoresis marker, ready to use DNA ladder, ready to use DNA marker
• NACRES: NA.25

Properties

• Quality Level: 200
• form: liquid
• usage: 100 uses
• suitability: suitable for electrophoresis (DNA)
• storage temp.: −20°C

Description

General description

Sigma′s DirectLoad^™ 1 kb Ladder contains 11 fragments consisting of 500 bp repeats from 0.5 to 3 kb, 1 kb repeats from 3 to 6 kb, and 2 kb repeats from 6 to 10 kb. For NA electrophoresis, 5 ul of the marker can be loaded directly into a single lane on an agarose or polyacrylamide gel. Suitable for use in Northern and Southern blotting.

Application

Suitable for size determination of dsDNA by DNA electrophoresis with either agarose or polyacrylamide gels.

Features and Benefits

• Ready-to-load
• Easy-to-use
• Popular band sizes

Components

Sigma′s DirectLoad^™ 1kb Ladder is provided in a gel-loading solution of 2.5% Ficoll (Type 400), 0.0125% bromophenol blue, and 0.00625% xylene cyanol.

Other Notes

Contains 11 fragments consisting of 500 bp repeats from 0.5 to 3 kb, 1 kb repeats from 3 to 6 kb and 2 kb repeats from 6 to 10 kb.

For optimal resolution, the recommended agarose gel concentration is 0.75%.

Legal Information

DirectLoad is a trademark of Sigma-Aldrich Co. LLC

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable