D8406
Anti -Dinitrophenyl antibody, Mouse monoclonal
IgE isotype, ~1 mg/mL, clone SPE-7, affinity purified immunoglobulin, buffered aqueous solution
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biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
affinity purified immunoglobulin
antibody product type
primary antibodies
clone
SPE-7, monoclonal
form
buffered aqueous solution
concentration
~1 mg/mL
technique(s)
indirect ELISA: 0.05-0.1 μg/mL using using 0.5 μg/well DNP-BSA as the coating antigen and 1 μg/mL Monoclonal Anti-Mouse IgE, Peroxidase conjugate as the detector antibody.
isotype
IgE
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
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General description
IgE gene is encoded on human chromosome 14. IgE is the least circulating antibody, but critical for eliciting hypersensitivity reaction against allergens.
Immunogen
Application
Western Blotting (1 paper)
Monoclonal antidinitrophenyl specific mouse IgE has been used in degranulation of rat mast cells using microplate reader and fluorescence spectrophotometer.
Biochem/physiol Actions
Physical form
Storage and Stability
Disclaimer
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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