DUO94002
Duolink® flowPLA Detection Kit - Green
Duolink® PLA kit for Flow Cytometry with Green Detection
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product line
Duolink®
technique(s)
flow cytometry: suitable
immunofluorescence: suitable
proximity ligation assay: suitable
fluorescence
λex 495 nm; λem 527 nm
suitability
suitable for fluorescence
shipped in
dry ice
storage temp.
−20°C
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This Item | DUO94001 | DUO94003 | DUO94005 |
|---|---|---|---|
| product line Duolink® | product line Duolink® | product line Duolink® | product line Duolink® |
| technique(s) flow cytometry: suitable, immunofluorescence: suitable, proximity ligation assay: suitable | technique(s) flow cytometry: suitable, immunofluorescence: suitable, proximity ligation assay: suitable | technique(s) flow cytometry: suitable, immunofluorescence: suitable, proximity ligation assay: suitable | technique(s) flow cytometry: suitable, immunofluorescence: suitable, proximity ligation assay: suitable |
| fluorescence λex 495 nm; λem 527 nm | fluorescence λex 594 nm; λem 624 nm | fluorescence λex 554 nm; λem 576 nm | fluorescence λex 390 nm; λem 476 nm |
| suitability suitable for fluorescence | suitability suitable for fluorescence | suitability suitable for fluorescence | suitability suitable for fluorescence |
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in dry ice |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
Specificity
Use appropriate laser for λex 495 nm excitation
Use appropriate filter for λem 527 nm emission
Application
Primary antibodies are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC), or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Flow validated antibodies are recommended.
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Duolink® flowPLA Detection Kits will enable sensitive detection of proteins, protein-protein interactions, and protein modifications within cell populations by flow cytometry. To perform a Duolink® flowPLA experiment, you will need fixed, suspended cells, two primary antibodies that specifically recognize your proteins of interest, a pair of PLA probes (one 100RXN PLUS and one 100RXN MINUS), wash buffer, and a Duolink® flowPLA Detection Kit. The flowPLA Kits are available with 5 different fluorophores: Violet, Red, Green, Orange, or FarRed. The flowPLA Kits contain all the necessary reagents to perform the amplification and detection of bound PLA probes by flow cytometry. Analysis is carried out using standard flow cytometry assay equipment. User must provide a fixed cell suspension, primary antibodies, and corresponding PLA Probes.
Follow the Duolink® PLA Flow Cytometry Protocol to use this product.
Visit our Duolink® PLA Flow Cytometry page on how to run a Duolink® flow experiment, applications, troubleshooting, and more.
Features and Benefits
- Analyze protein protein interactions with flow cytometry readout
- Analyze cell populations with Proximity Ligation Assay
- Increased sensitivity due to rolling circle amplification for low abundant targets
- No overexpression or genetic manipulation required
- Relative quantification possible
- Works with any flow cytometer instrumentation
- Easy to follow flexible protocol
- Publication-ready results
Components
- 5x Detection Solution - Green (DUO84002)
- 5x Ligation Buffer (DUO82009)
- 5x Amplification Buffer (DUO82050)
- Ligase (1U/μL)
- Polymerase (10U/μL)
See datasheet for more information.
Legal Information
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
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