MAK478
α-Amylase Activity Assay Kit
sufficient for 100 colorimetric tests
Synonym(s):
Assay Kit for Amylase Activity
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About This Item
Recommended Products
Quality Level
application(s)
agriculture
food and beverages
detection method
colorimetric
relevant disease(s)
endocrinological disorders; gastrointestinal diseases; genitourinary disorders
shipped in
wet ice
storage temp.
−20°C
Related Categories
General description
Amylase belongs to the family of glycoside hydrolase enzymes that break down starch into glucose molecules by acting on α-1,4- glycosidic bonds. The α-amylases cleave at random locations on the starch chain, ultimately yielding maltotriose and maltose, glucose and "limit dextrin" from amylose and amylopectin. In mammals, α-amylase is a major digestive enzyme. Increased enzyme levels in humans are associated with salivary trauma, mumps due to inflammation of the salivary glands, pancreatitis, and renal failure.
Features and Benefits
- The linear detection range of the kit is 0.3 to 50 U/L α-amylase.
Suitability
The kit is suitable for the determination of α-amylase activity in blood, saliva, urine, grains, and other agricultural samples.
Principle
Simple, direct, and automation-ready procedures for measuring amylase activity are very desirable. The α-Amylase Activity Assay Kit method involves two steps. First, α -amylase in the sample hydrolyses starch and the product is rapidly converted to glucose by α-glucosidase and hydrogen peroxide by glucose oxidase. Next, the hydrogen peroxide concentration is determined with a colorimetric reagent at 585 nm.
WGK
WGK 2
Certificates of Analysis (COA)
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