Skip to Content
Merck
All Photos(1)

Documents

MAK482

Sigma-Aldrich

Lipase Assay Kit

sufficient for 100 colorimetric tests

Synonym(s):

Lipase Activity Assay Kit

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
12352204
NACRES:
NA.84

Quality Level

detection method

colorimetric

relevant disease(s)

genitourinary disorders; gastrointestinal diseases; cancer

shipped in

ambient

storage temp.

2-8°C

Related Categories

General description

Lipase catalyzes the hydrolysis of ester bonds on the glycerol backbone of a lipid substrate. In humans, pancreatic lipase is the key enzyme responsible for breaking down fats in the digestive system by converting triglycerides to monoglycerides and free fatty acids. Human pancreatic lipase and its related protein 2 are the main lipases secreted by the pancreas. In acute pancreatitis, lipase levels can rise 5 to 10-fold within 24 to 48 hours. Increased activities have also been associated with pancreatic duct obstruction, pancreatic cancer, kidney disease, salivary gland inflammation, bowel obstruction, and other pancreatic diseases. Decreased levels may indicate permanent damage to lipase-producing cells in the pancreas.

Features and Benefits

  • The linear detection range of the kit is 40 to 1600 U/L lipase

Suitability

The kit is suitable for detecting lipase activity in serum, plasma, saliva, urine and other biological samples.

Principle

The Lipase Assay Kit is based on an improved Dimercaptopropanol tributyrate (BALB) method, in which SH groups formed from lipase cleavage of BALB react with 5,5′-dithiobis (2-nitrobenzoic acid) (DTNB) to form a yellow-colored product. The color intensity, measured at 412 nm, is proportionate to the enzyme activity in the sample.

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Flam. Liq. 2 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

WGK

WGK 3


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service