Skip to Content
Merck
All Photos(1)

Documents

N5665

Sigma-Aldrich

BLOCKED P-NITROPHENYL-ALPHA-D-MALTOHEPTAOSIDE

Sign Into View Organizational & Contract Pricing

Synonym(s):
BpNPG7
UNSPSC Code:
12352200
NACRES:
NA.28

form

powder

Quality Level

mol wt

1362

impurities

≤3% Water content (Karl Fischer)

color

white to yellow

shipped in

dry ice

storage temp.

−20°C

General description

This product is useful as a substrate for determination of α-amylase activity. This oligosaccharide contains a blocking group at the non-reducing end, which prevents exoglucosidase from attacking the substrate molecule. Amylase can still cleave the substrate internally, which releases short p-nitrophenol-linked (pNP-linked) oligosaccharides. After removal of the blocking group, auxiliary enzymes such as glucoamylase and maltase can release the chromogen. Glucoamylase accelerates this reaction via action on the longer chains. Maltase then separates these longer chains from the pNP moiety.

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

DNA typing after α-amylase test.
Rapone, C. et al.
International Congress Series, 1239, 911-915 (2003)
Bruna Yuki Tagomori et al.
3 Biotech, 11(1), 13-13 (2021-01-15)
This work reports the amy1 gene cloning from Massilia timonae CTI-57, and its successful expression in Escherichia coli Rosetta™ (DE3) from the pTRCHis2B plasmid. The recombinant AMY1 protein had 47 kDa, and its modeled structure showed a monomer composed of three

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service