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Nucleic Acid Sample Preparation Methodology
Spectrophotometry can be used to estimate DNA or RNA concentration and to analyze the purity of the preparation.
Whole Genome Amplification from Serum or Plasma Protocol
Whole genome amplification (WGA) of plasma and serum DNA presents a unique challenge due to the small amount of nucleic acid in such samples.
Protector RNase Inhibitor Protocol & Troubleshooting
The addition of Protector RNase Inhibitor to cell pellets is not recommended. Protector RNase Inhibitor as a 50kDa protein will most probably not permeate into intact cells. If the cell pellet is immediately frozen at -80 °C, this will be
Instructions for EasiCollect™
This document shows intructions for EasiCollect™ device from Cytiva.
RNA Stabilization at Room Temperature in RNAstable®
Quantitative RT-PCR analysis demonstrates successful amplification of RNA templates that were stored dry in RNAstable® for 29 months at room temperature and under accelerated aging conditions equivalent to 12 years of room temperature storage.
Biological Sample Collection, Transport, Archiving, and Purification for Nucleic Acid Analysis
A number of options are available for sample collection, transport, archiving, and DNA purification.
Genomic DNA Purification using illustra™ GenomicPrep Midi Flow Kits
This page shows Genomic DNA purification using illustra™ genomicPrep Midi Flow Kits from Cytiva.
Buccal DNA Extraction & WGA Amplification Protocol
This protocol provides a simple and convenient method to isolate, amplify, and purify genomic DNA from buccal swabs. Buccal swabs are a convenient method of acquiring a DNA sample.
GenElute™ Mammalian Genomic DNA Miniprep Kit Protocol
The GenElute Mammalian Genomic DNA Purification Kit Protocol describes the isolation of pure, high molecular weight DNA from a variety of mammalian sources.
Plant-Based PCR Inhibitors During DNA Extraction and PCR Master Mix Performance
Evaluating the effects of PCR inhibitors and PCR enzyme selection using plant genomic DNA, GenElute™-E Single Spin Plant Extraction Kit, KOD-One™ PCR Master Mix and competitor high fidelity PCR master mixes.
Animal Tissue DNA-Extraction & WGA Amplification Protocol
GenomePlex® Whole Genome Amplification is the method of extracting DNA from the animal sample. GenomePlex® products have been used to amplify genomic DNA from chicken, porcine, bovine, fish, and shrimp source.
GenElute™ Plant Genomic DNA Miniprep Kit Protocol (G2N10, G2N70, G2N350)
This protocol describes a simple and convenient procedure to isolate pure DNA from a variety of plant species using the GenElute Plant Genomic DNA Miniprep Kit.
REDExtract-N-Amp™ Plant PCR Kit Protocol
The REDExtract-N-Amp Plant PCR kit includes an inert loading dye in the ReadyMix for easy gel loading after amplification. This protocol provides a rapid protocol to extract plant genomic DNA for amplification using the optimized ReadyMix.
Extract-N-Amp™ Plant PCR Kits Protocol
The Extract-N-Amp™ Plant PCR Kits contain all of the reagents required to rapidly extract and amplify genomic DNA from plant leaves.
Whole Gene Amplification Formalinfixed Paraffin embedded Tissues
Archived formalin-fixed and paraffin-embedded (FFPE) tissue collections represent invaluable resources for studying pathogenesis of cancer and a variety of other diseases.
Extract-N-Amp™ FFPE Tissue PCR Protocol
Protocol for PCR amplification of formalin-fixed paraffin embedded animal tissues using the Extract-N-Amp Tissue PCR kit.
SYBR® Green Extract-N-Amp™ Tissue PCR Kit Protocol
The SYBR® Green Extract-N-Amp™ Tissue PCR Kit contains all the reagents needed for rapid extraction, amplification and detection of genomic DNA from mouse tails and other animal tissues, buccal swabs, hair shafts, and saliva.
Extract-N-Amp™ FFPE Tissue PCR Protocol
The following procedure is a modification of the Extract-N-Amp™ Tissue PCR protocol.
DNase I Recombinant, RNase-free Protocol
DNase I from bovine pancreas is a glycoprotein of Mr 37000. A special procedure is used to remove RNases from the DNase preparation.
Oligonucleotide Handling & Stability
Handing and stability techniques to reduce variation and ensure trouble-free experiments.
FFPE Tissue
Archived Formalin-fixed, Paraffin-embedded (FFPE) tissue samples are invaluable resources for profiling gene expression and studying a variety of diseases.
Saliva DNA Extraction & WGA Amplification Protocol
Whole Genome Amplification can be performed on DNA extracted in many ways. We offer many products for DNA extraction, including the GenElute™ Blood Genomic DNA Kit, GenElute Mammalian Genomic DNA Miniprep Kit and the GenElute Plant Genomic DNA M iniprep.
Plasmid DNA Preparation
Preparation of Plasmid DNA by Alkaline Lysis with SDS: Maxipreparation between Cold Spring Harbor Laboratory Press and our research team.
GenElute™ RNA/DNA/Protein Plus Purification Kit
GenElute RNA/DNA/Protein Plus Purification Kit provides a rapid method for the isolation and purification of total RNA, genomic DNA and proteins sequentially from a single sample of cultured animal cells, small tissue samples, blood, bacteria, yeast, fungi or plants.
Extract-N-Amp™ Tissue PCR Kit Protocol
The Extract-N-Amp™ Tissue PCR Kit contains all the reagents needed to rapidly extract and amplify genomic DNA from mouse tails and other animal tissues, buccal swabs, hair shafts, and saliva.
Spermine Tetrahydrochloride for Molecular Biology
This product is suitable for DNA precipitation from low salt aqueous buffers. It has been tested for the absence of proteases and nucleases.
Selecting Correctly Expressing Recombinants
Blue White Screening; DNA Minipreps; Screening by Restriction Digestion; Screening by PCR; Confirm cut plasmid sizes by agarose gel electrophoresis; DNA Maxipreps; DNA Precipitation; RNase Treatment; Clean-up of DNA
GenElute™ Bacterial Genomic DNA Kit Protocol (NA2100, NA2110, NA2120)
GenElute™ Bacterial Genomic DNA Kit protocol describes a simple and convenient way for the isolation of pure genomic DNA from bacteria.
Genomic DNA Extraction and Amplification Procedure using REDExtract-N-Amp™ Tissue PCR Kit
The REDExtract-N-Amp™ Tissue PCR Kit contains all the reagents needed to rapidly extract and amplify genomic DNA from mouse tails and other animal tissues, buccal swabs, hair shafts, and saliva.
GenElute™ 96 Well Tissue Genomic DNA Purification Kit Protocol
This protocol is designed to allow for high throughput purification of pure genomic DNA from a variety of cultured cells and tissues using the GenElute 96 Well Tissue Genomic DNA Purification Kit.
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