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An ultrafiltration cartridge can be placed at the outlet of a water purification system to deliver nuclease-free ultrapure water.

Plasmid DNA Preparation Troubleshooting

This page shows possible causes and solutions for problems that may occur during plasmid DNA preparation.

Oligonucleotide Quality Control by Analytical HPLC

Stabilizing RNA at room temperature in RNAstable®

RNAstable® is a novel preservation product developed to protect RNA from degradation during storage or shipment at ambient temperatures. The synthetic storage medium is based on the natural principles of anhydrobiosis (meaning “life without water”), a biological mechanism employed by

ECACC Nucleic Acids

DNA, RNA, cDNA derived from ECACC mammalian cell lines allow screening for genes or expression patterns to identify lines most suitable for specific research.

Lentiviral Packaging

The goal of this exercise is to learn lentiviral packaging. For this example we will be using the HEK 293T cell line.

Extract-N-Amp™ Blood PCR Kit Protocol

The Extract-N-Amp Blood PCR Kits contain all the reagents needed to rapidly extract and amplify human genomic DNA from whole blood, whole blood dried on a blood card, and cultured mammalian cells.

Protein Affinity Chromatography

Affinity chromatography is the process of bioselective adsorption and subsequent recovery of a compound from an immobilized ligand. This process allows for the highly specific and efficient purification of many diverse proteins and other compounds. The process requires

Sodium acetate buffer solution for molecular biology

Sodium Acetate Buffer provider. 5+ decades providing high-quality buffer products that maintain consistent performance for molecular biology .

Procedure for Purifying and Concentrating DNA using illustra™ GFX 96 PCR Purification Kit

This page shows the procedure for purifying and concentrating DNA using illustra™ GFX 96 PCR Purification Kit from Cytiva.

Extract-N-Amp™ Plant Tissue PCR Kits

The Extract-N-Amp™ kits are designed to rapidly extract and amplify genomic DNA. The plant tissue version of these kits has been optimized to amplify without concern over plant inhibitors. This technical document will discuss the versions of this kit that

High Pure Technology and Silica Adsorption Kits

The High Pure and silica adsorption kits developed by Roche depend on the tendency of nucleic acids to adsorb to silica (glass) in the presence of a chaotropic salt such as sodium iodide (NaI), guanidine thiocyanate or guanidine hydrochloride [Melzak

RNA Preparation Troubleshooting

This page shows possible causes and solutions for problems that may occur during RNA preparation.

RNA Isolation from Blood, Cultured Cells, and Animal or Plant Tissue on Whatman™ FTA™ Cards

This article describes the evaluation of Whatman FTA cards from Cytiva for their ability to collect, store, and isolate high-quality RNA from a variety of crude biological samples.

Impact of GenElute™-E Purification Method on Accuracy of DNA Quantitation and Downstream Enzymatic Processes

Impact of Purification Method on Accuracy of DNA Quantitation and Downstream Enzymatic Processes. Evaluation of the purity of genomic DNA by UV spectrophotometry, gel electrophoresis, and downstream qPCR using GenElute™-E DNA purification kits.

Benzonase^® Nuclease for Microbiome Workflows

Benzonase® Nuclease for reducing host DNA in microbiome workflows and enhancing taxa identification.

DNA and RNA Quantification

An overview of methods for quantifying DNA and RNA, how to measure DNA and RNA concentration and yield, and how to assess purity of nucleic acid samples.

Biomatrica® offers an innovative alternative to reliance on cold chain logistics

Biomatrica® products enable researchers to stabilize, ship, and store biological samples for long periods of time.

Sera-Mag™ and Sera-Mag SpeedBeads Magnetic Particles

Sera-Mag and Sera-Mag SpeedBeads provide cost effective magnetic bead separation technology for molecular biology applications, nucleic acid isolation, and research immunoassays.

Long-term Storage of Blood in DNAgard® Blood

Two separate studies were conducted to analyze long-term storage of blood in DNAgard Blood stabilization reagent.

Extract-N-Amp Reagent Provides a Highly Effective Way of Generating PCR Products from FTA® Blood Cards

Extract-N-Amp is a versatile, combined DNA extraction and amplification kit intended to simplify the generation of PCR products from a range of sample types. This methodology can be used without modification to perform PCR on blood samples stored on FTA

Histone Modification and Chromatin Remodeling | Epigenetics

Epigenetic modifications are thought to occur through two key interconnected processes—DNA methylation and the covalent modification of histones.

Extract-N-Amp™ Tissue Feature Article

Standard methods for extracting DNA from tissues can be extremely laborious and time consuming. Certain applications, such as genotyping of transgenic mice using a section of tail, employ a lengthy DNA extraction process.

Downstream Applications for Plasmid DNA and Substrates

This page shows downstream applications for plasmid DNA and substances that may interfere in each.

General Considerations for Plasmid DNA Preparation

This page discusses methods for plasmid DNA purification and the factors impacting resulting DNA purity and yield using illustra plasmidPrep Kits from Cytiva.

Downstream Applications after Genomic DNA Purification

This page shows downstream applications after genomic DNA purification.

Complete Solutions for PCR Assay Development

Fit-for-use products offer the quality, consistency & documentation necessary for every step of your IVD development and manufacturing process.

Viral RNA Extraction Buffer for SARS-CoV-2 in Saline and Saliva

Viral RNA Extraction Buffer (VRE100) and related reagents for qRT-PCR and additional viral RNA analyses.

MetaPolyzyme, DNA Free Cell Lysis Enzymes for Microbiome Workflows | Sigma-Aldrich

Explore the benefits of MetaPolyzyme, DNA free to improve DNA extraction and increase the number of taxa identified in treated samples.

KAPA Express Extract Kit FAQs

Frequently asked questions (FAQs) for KAPA Express Extract Kit.

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