Enzyme activity assays

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Applications:Enzyme activity assays

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Derived from procedure SSPHYT02. Includes template updates to current SOP specifications and incorporation of notes into the procedure.

Enzymatic Assay of Alcohol Oxidase (EC 1.1.3.13)

This procedure may be used for all Alcohol Oxidase products. The continuous spectrophotometric rate determination (A405, Light path = 1 cm) is based on the following reactions.

Enzymatic Assay of Esterase

Objective: To standardize a procedure for the enzymatic determination of Esterase activity using Ethyl Butyrate as a substrate.

Enzymatic Assay of Acid Phosphatase (EC 3.1.3.2)

Procedure for Enzymatic Assay of α-Chymotrypsin (EC 3.4.21.1)

Follow our procedure for the determination of a chymotrypsin activity. This enzymatic assay of alpha chymotrypsin guides you through the entire process and necessary calculations.

Assay Procedure for Protease

This procedure is for informational purposes including assay Procedure, definition, and calculations for Protease

GST Detection Module with CDNB Enzymatic Assay

This page describes enzymatic detection of GST-tagged proteins with the GST Detection Module from Cytiva.

Enzymatic Assay of Diamine Oxidase (E.C. No. 1.4.3.22)

To standardize a procedure for the enzymatic assay of Diamine Oxidase.

Enzymatic Assay of Achromopeptidase

Enzymatic Assay of Pepsin (3.4.23.1)

This procedure may be used for determination of Pepsin activity using hemoglobin as the substrate. It is a spectrophotometric stop rate determination.

IFN-γ ELISpot Assays on MultiScreen® IP

The ELISpot (Enzyme Linked Immuno-Spot) assay provides an effective method of measuring the antibody or cytokine production of immune cells on the single cell level.

Enzymatic Assay of Amyloglucosidase (EC 3.2.1.3)

This procedure may be used for the determination of Amyloglucosidase activity using starch as the substrate.

Enzymatic Assay of Carboxypeptidase

Objective: To standardize a procedure for the assay of Carboxypeptidase A

Enzymatic Method for Determining Glucose-6-Phosphate (Glucose-6-Phosphate Assay)

Enzymatic Activity of Glucose-6-Phosphatase [EC 3.1.3.9]

Objective: To standardize a procedure for the enzymatic determination of Glucose-6-phosphatase activity

Enzymatic Assay of L-Lactic Dehydrogenase (EC 1.1.1.27)

This procedure applies to all products from heart muscle that have a specification for L-Lactic Dehydrogenase activity.

Enzymatic Assay of Laccase (EC 1.10.3.2)

Laccase (EC 1.10.3.2) is a cuproenzyme that oxidizes various types of phenols and similar aromatic compounds aromatic amines with the reduction of molecular oxygen to water, therefore, is used as a biocatalyst.

Enzymatic Assay of Collagenase (EC 3.4.24.3) using FALGPA (N-(3-[2-Furyl]acryloyl)-Leu-Gly-Pro-Ala)

This procedure may be used for Collagenase products.

Enzymatic Assay of Lysozyme (EC 3.2.1.17)

This enzymatic rate determination may be used for Lysozyme products. It is not to be used to assay recombinant or insoluble Lysozyme on agarose.

Protocol Guide: WST-1 Assay for Cell Proliferation and Viability

WST-1 assay protocol for measuring cell viability, proliferation, activation and cytotoxicity. Instructions for WST-1 reagent preparation and examples of applications. Frequently asked questions and troubleshooting guide for WST-1 assay.

Enzymatic Assay of Sulfatase (EC 3.1.6.1.)

Recycling Micro-Assay of β-NAD and β-NADH

Objective: To standardize a procedure for the recycling micro-assay of β-NAD and β-NADH

Assay Procedure for Glycerol Dehydrogenase

Enzymatic Assay of Ferredoxin NADP Reductase (EC 1.18.12)

Enzymatic Assay of Ferredoxin NADP Reductase - To standardize a procedure for the enzymatic assay of Ferredoxin NADP Reductase (EC.1.18.12).

Procedure for Enzymatic Assay of Trypsin (EC 3.4.21.4)

This procedure is for products with a specification for Trypsin activity using Na-Benzoyl-L-arginine ethyl ester (BAEE) as a substrate. The procedure is a continuous spectrophotometric rate determination (A253, Light path = 1 cm).

Enzymatic Assay of Peroxidase (EC 1.11.1.7)

This procedure is for the determination of Peroxidase enzymatic activity using Pyrogallol as the substrate.

Enzymatic Assay of Deoxyribonuclease I (EC 3.1.21.1)

To standardize a procedure for the enzymatic assay of Deoxyribonuclease I.

Enzymatic Assay of Trypsin Inhibitor

This technical article described the Enzymatic Assay of Trypsin Inhibitor.

Enzymatic Assay of β-Glucuronidase (EC 3.2.1.31) from E. coli

Objective: To standardize a procedure for the enzymatic assay of β-Glucuronidase.

Enzymatic Assay of Pectinase

Objective: To standardize a procedure for determining the enzymatic activity of Pectinase.

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