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DIG Gel Mobility Shift Assay Troubleshooting
To prevent nonspecific binding of the analyzed protein or extract to the DNA fragment or oligonucleotide, nonspecific competitor nucleic acid is added to the binding reaction.
ChIP-qPCR and Data Analysis
Chromatin Immunoprecipitation quantitative real-time PCR (ChIP-qPCR) is commonly used in studies that focus on specific genes and potential regulatory regions across differing experimental conditions and data analysis. qPCR enables DNA analysis in real time by analyzing fluorescent signal intensities that
Imprint® Chromatin Immunoprecipitation Kit (CHP1) Protocol
The Sigma Imprint Chromatin Immunoprecipitation Kit uses a plate based system to allow rapid ChIP assays in a high throughput format
Chromatin Isolation by RNA Purification (ChIRP) Protocol
Chromatin Isolation by RNA purification (ChIRP) is a method for isolating regions of the genome that are bound by a specific RNA. This protocol uses magnetic beads coated with streptavidin to isolate the complex.