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Quantification of Infliximab in Human Serum by LC-MS/MS Using A Full-Length Stable Isotope Labeled Internal Standard
Infliximab, a chimeric monoclonal antibody, is used to treat rheumatoid arthritis, psoriatic arthritis, Crohn’s disease, and other autoimmune diseases.
Absolute Quantification of Serum Proteome
Absolute Quantification (Protein-AQUA™™) is a targeted quantitative proteomics technique that exhibits robust efficacy and is being increasingly utilized for a wide variety of quantitative proteomics studies.
Peptide Sample Amount Determination
Determining the amount of desired product in a peptide sample.
Ascentis® Express Phenyl Hexyl Columns for U/HPLC
Improve your reversed phase selectivity for polar aromatics and heterocyclic compounds with the Ascentis® Express Phenyl-Hexyl U/HPLC column.
Chromolith® HPLC and UHPLC columns
Chromolith® HPLC and UHPLC columns are made from monolithic silica with a bimodal pore structure using sol-gel technology.
Ascentis® Express C18 U/HPLC Columns
Ascentis® Express C18 is the first choice when starting a new separation method.
How to Select the Right Water Purification System for Your Lab
Technical article on Selecting the Right Water Purification System
LC/MS (TOF) Analysis of Antiarrhythmic Drugs and Metabolites on Ascentis® Express HILIC
HILIC mobile phases consist of a high composition of acetonitrile, which facilitates the direct analysis of precipitated plasma samples without the need for additional sample solvent exchange.
HPLC Analysis of Z-Drugs on Ascentis® Express ES-Cyano 2.0 μm
An rapid method for the siµLtaneous determination of the Z-drugs or sleep aids: zopiclone, zolpidem, and zaleplon is presented here. The need for greater analytical capacity and throughput for the analysis of sleep aid medicines (Z-drugs) in forensic toxicology laboratories
High-Resolution U/HPLC Separation of Monoclonal Antibodies with the BIOshell™ IgG 1000 Å Diphenyl
BIOshell™ IgG 1000 Å Diphenyl columns are perfectly suitable for the separation of monoclonal antibodies, antibody-drug conjugates, antibody fragments and large proteins with molecular weights ≤ 500 kDa with alternative selectivity to C4 and C18 column chemistries to ensure maximum
Dyestuff Analyses on Historic Objects by LC-ESI-MS/MS Using a Superspher RP-18 HPLC Column
In this study, LC-ESI-MS/MS under reversed phase conditions using a Superspher RP-18 column is applied to analyze dyestuffs of a reddish historic textile from South America.
LC/MS Analysis of Ethanol Metabolites in Urine on Ascentis® Express OH5 after Dilution with Deuterated Internal Standards
The method shown uses HILIC mode on an Ascentis® Express OH5 column to retain both analytes well, making it very likely to be more robust and reliable, as well as highly MS-friendly.
UHPLC/MS Analysis of Diazepam and Metabolites on Titan™ C18, Comparison of Flow Rate and Column Length
Separation of Oxazepam; Desmethyldiazepam, analytical standard; Temazepam, analytical standard; Diazepam
HPLC Analysis of Methylphenobarbital (Mephobarbital) Enantiomers on Astec® CHIROBIOTIC® V
Separation of Mephobarbital, United States Pharmacopeia (USP) Reference Standard
UHPLC Analysis of Conjugated Estrogens on Ascentis ® Express C18
Separation of Estriol 3-(β-D-glucuronide) sodium salt; β-Estradiol 3-(β-D-glucuronide) 17-sulfate dipotassium salt; Estriol 3-sulfate sodium salt; β-Estradiol 3,17-disulfate dipotassium salt, ≥95%; β-Estradiol 17-(β-D-glucuronide) sodium salt; β-Estradiol 3-(β-D-glucuronide) sodium salt; Estrone 3-(β-D-glucuronide) sodium salt; β-Estradiol 3-sulfate sodium salt, ≥93%; Estriol, ≥97%; Estrone
Determination of Sucralose in Soy Sauce using HPLC-ELSD following the Chinese National Standard (GB) Method
The most frequently used synthetic sweeteners are: saccharin, cyclamate, aspartame, and sucralose (E955), and this application illustrate the analysis of sucralose from soy sauce following the current Chinese national standard method.
HPLC Analysis of Sibutramine Enantiomers on Astec® CHIROBIOTIC® V
Separation of Sibutramine hydrochloride monohydrate, ≥98% (HPLC), solid
Determination Paeonol Morroniside Loganin Liuwei Dihuang Oral Solution
Method development for the extraction and analysis of paeonol, morroniside, loganin active ingredients in traditional Chinese medicine (TCM) according to Chinese pharmacopeia monograph using HPLC-UV
HPLC Tips & Tricks: Mobile Phase Preparation Part 2 - Buffers
Learn the tips and tricks of selecting and preparing a suitable mobile phase buffer for your HPLC separations and method developments for samples that contain ionizable compounds.
HPLC analysis of Letrozole and its impurities by following the USP Guidelines Using a Purospher® STAR RP18e Column and UV Detection
An application note concerning the HPLC analysis of letrozole and its impurities by following the USP43-NF38 Guidelines using a Purospher® STAR RP18e Column.
Determination of Chlorogenic acid and Baicalin in compounded Lonucerae Japonicae Flos with Purospher® STAR RP-18e
Extraction and analysis of chlorogenic acid and baicalin active ingredients in traditional Chinese medicine (TCM) according to Chinese pharmacopeia monograph by HPLC with Purospher® RP-18e column
Columns and Analytical Standards for Protein SEC
Size-exclusion chromatography (SEC) columns and ready-to-use standards facilitate method development and increase robustness of protein SEC methods.
HPLC Analysis of Proteins on BIOshell™ A400 Protein C4, Rapid Separation at Elevated Temperature
For use as a marker in SDS-PAGE; lactate dehydrogenase; contaminant; L-Lactic Dehydrogenase from rabbit muscle, Type XI, lyophilized powder, 600-1,200 units/mg protein
HPLC Analysis of Sugars by HILIC Chromatography using Ascentis® Express OH-5 Column with ELSD Detection
Separation of Dextrose, Pharmaceutical Secondary Standard; Certified Reference Material; Sucrose; Maltitol, Pharmaceutical Secondary Standard; Certified Reference Material
High Resolution and High Efficiency Separations of mAbs and ADCs Using Proteomix HIC Columns
Hydrophobic interaction chromatography, a protein separation technique, has been commonly used as an orthogonal method to SEC and ion exchange for the characterization of mAbs.
HPLC determination of Besifloxacin in Ophthalmic Suspension using Chromolith® SpeedROD RP-18e Column
A new HPLC method is proposed for the determination of Besifloxacin, a fluoroquinolone antibiotic in ophthalmic suspension using a Chromolith® SpeedROD RP-18e column. Read now!
HPLC Analysis of Caseins on BIOshell™ A400 Protein C4
-casein basis (electrophoresis), lyophilized powder; β-Casein from bovine milk, BioUltra, ≥98% (PAGE)
HPLC Analysis of Pain Relievers on Ascentis® Express C18
Separation of Acetaminophen, meets USP testing specifications, 98.0-102.0%, powder; Caffeine, meets USP testing specifications, anhydrous; Benzoic acid, meets USP testing specifications; Aspirin, meets USP testing specifications
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