Protein labeling and modification

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Our protein quality supplement, EX-CELL® Glycosylation Adjust (Gal+), provides customers with a novel chemically defined product which targets glycosylation attributes.

Enzymes for Antibody Characterization

Antibodies now make up the fastest growing category of therapeutic drugs

Troubleshooting Strategies in GST-tagged Protein Purification

This page describes troubleshooting strategies in purification of GST-tagged proteins using Cytiva products.

Utilizing ISOGRO® Supplementation of M9 Minimal Media to Enhance Recombinant Protein Expression

Utilizing ISOGRO® Supplementation of M9 Minimal Media to Enhance Recombinant Protein Expression.

Chymotrypsin

Analytical Enzyme Chymotrypsin: Chymotrypsin is produced in the acinar cells of the pancreas as the inactive precursor, chymotrypsinogen.

Cleavage and Purification of GST-Tagged Protein Bound to Glutathione Sepharose in Batch Mode

This page shows how to cleave and purify GST-tagged proteins bound to Glutathione Sepharose in batch mode from Cytiva. Glutathione Sepharose High Performance, Glutathione Sepharose 4 Fast Flow, and Glutathione Sepharose 4B can all be used for cleavage and purification

Peptide Modifications: N-Terminal, Internal, and C-Terminal

Cleavage and Purification of GST-Tagged Protein Bound to GSTrap

This page shows how to cleave and purify GST-tagged proteins bound to GSTrap from Cytiva.

ChiPros Chiral Amines

Chiral amines play an important role in stereoselective organic synthesis. They are used directly as resolving agents, building blocks, or chiral auxiliaries.

Mass Spectrometry

What is Mass Spectrometry or MS? What is it used for and how does it work? Read this detailed article with diagrams and example graphs to understand how to use mass spec in your research.

Chiral Amines in Asymmetric Synthesis

Chiral amines have found widespread application in asymmetric synthesis serving, for instance, as chiral bases in enantioselective deprotonation reactions or being valuable substances for resolving racemic mixtures of acids.

Unnatural Amino Acids for Peptide Synthesis

Unnatural amino acids, the non-proteinogenic amino acids that either occur naturally or are chemically synthesized, are becoming more and more important as tools for modern drug discovery research.

Degrader Building Blocks for Targeted Protein Degradation

Protein Degrader Building Blocks are a collection of crosslinker-E3 ligand conjugates with a pendant functional group for covalent linkage to a target ligand.

Proline Derivatives and Analogs

Proline analogues are promising candidates for tuning the biological, pharmaceutical, or physicochemical properties of naturally occuring, as well as de novo designed, linear, and, cyclic peptides.

Glycosylphosphatidylinisotol-anchored Glycoproteins Overview

GPI Anchored Glycoproteins

Improving Glycoform Coverage for Mucin Prep

Human Protein Atlas uses A-431, U-251 MG, and U-205 cell lines with Prestige Antibodies for organelle mapping.

Designing Peptides

Consider sequence length, solubility, and stability when designing peptides for custom synthesis.

Post Translational Modification

Protein modifications are crucial for disease study. Analysis methods are key.

Strategies for Deglycosylating N-Linked Glycans

Explore strategies for releasing N-linked glycans with PNGase F, PNGase A & native & sequential deglycosylation with endoglycosidases & exoglycosidases.

Introduction to Proximity Labeling

Novel method tags antibodies with photoreactive iridium for membrane protein labeling.

O Linked Glycans

O-Linked glycans attachment to peptides involves various residues, particularly GalNAc, known as mucin-type glycans.

Phosphorylation Analysis Tools

Phosphorylation's role in cell signaling pathways and protein modification.

Y-Shape Peg Derivatives in Drug Delivery

Immune system defends against disease by hydrolyzing foreign molecules; proteases, nucleases, liver, and kidneys are involved.

Glycosyltransferases

Enzymatic glycosyltransferase specificity challenges the one enzyme-one linkage concept.

Glycan Labeling

Glycans play a key role in protein structure and disease; representation on cell surfaces is the glycome.

Gold Nanoparticles: Properties and Applications

Gold (Au) nanoparticles have tunable optical and electronic properties and are used in a number of applications including photovoltaics, sensors, drug delivery & catalysis.

Near-infrared (NIR) Fluorescence Dyes

NIR Fluorescence Dyes

Signal Peptide Optimization: Effect On Recombinant Monoclonal IgG Productivity, Product Quality And Antigen-Binding Affinity

A signal peptide is a 5-30 amino acid (aa) peptide present at the N-terminus of secretory proteins.

Introduction to Target Identification with Hyas and Dione Kits

In cases where photoaffinity labeling (PAL) probes are ineffective, Hyas and Dione Kits provide an effective method for small molecule identification by using an Iridium photocatalyst tethered to the molecule under investigation.

N-Linked Glycans Overview

N-linked glycosylation, modification, and degradation

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