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Protein labeling and modification

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Applications:Protein labeling and modification
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Cell and Organelle Labeling with Fluorescent Antibodies
The Human Protein Atlas Program has carefully selected three different human cell lines, A-431 epidermoid carcinoma, U-251 MG glioblastoma and U-205 osteosarcoma, for organelle mapping of the proteome. As Prestige Antibodies are studied by immunofluorescence (IF) staining, three well-characterized organelle
EX-CELL® Glycosylation Adjust (GAL+)
Our protein quality supplement, EX-CELL® Glycosylation Adjust (Gal+), provides customers with a novel chemically defined product which targets glycosylation attributes.
Enzymes for Antibody Characterization
Antibodies now make up the fastest growing category of therapeutic drugs
O Linked Glycans
O-Linked glycans are usually attached to the peptide chain through serine or threonine residues. O-Linked glycosylation is a true post-translational event and does not require a consensus sequence. The most common type of O-linked glycans contain an initial GalNAc residue
Post Translational Modification
Post-translational modifications such as glycosylation, phosphorylation, and sulfation, to name a few, serve many functions. As a result, the analysis of proteins and their post-translational modifications is particularly important for the study of diseases where multiple genes are known to
Troubleshooting Strategies in GST-tagged Protein Purification
This page describes troubleshooting strategies in purification of GST-tagged proteins using Cytiva products.
Introduction to Proximity Labeling
A novel method has been developed to tag antibodies with a photoreactive iridium catalyst. Light activates the catalyst to convert a diazirine compound into a reactive carbene intermediate that can label membrane proteins in close proximity. The diazirine adds a
Utilizing ISOGRO® Supplementation of M9 Minimal Media to Enhance Recombinant Protein Expression
Utilizing ISOGRO® Supplementation of M9 Minimal Media to Enhance Recombinant Protein Expression.
Analytical Enzyme Chymotrypsin: Chymotrypsin is produced in the acinar cells of the pancreas as the inactive precursor, chymotrypsinogen.
Phosphorylation Analysis Tools
Phosphorylation is an important covalent post-translational modification (PTM) in cell signalling pathways. Protein phosphorylation is the reversible addition of a phosphate group to a protein or small molecule catalysed by protein kinases. Approximately one third of the 30,000 proteins encoded
Designing Peptides
When selecting peptides for custom synthesis, several important factors should be considered during the design process. These considerations include sequence length, solubility and sequence stability.
Cleavage and Purification of GST-Tagged Protein Bound to Glutathione Sepharose in Batch Mode
This page shows how to cleave and purify GST-tagged proteins bound to Glutathione Sepharose in batch mode from Cytiva. Glutathione Sepharose High Performance, Glutathione Sepharose 4 Fast Flow, and Glutathione Sepharose 4B can all be used for cleavage and purification
Strategies for Deglycosylating N-Linked Glycans
Explore various strategies for deglycosylating N-linked glycans involving PNGase F, PNGase A (Glycopeptidase A), and even native and sequential deglycosylation with endoglycosidases like Endoglycosidase H, Endoglycosidase F, and exoglycosidases.
Glycan Labeling
Structural modifications of proteins are essential to living cells. When aberrantly regulated they are often the basis of disease. Glycans are responsible for much of the structural variation in biologic systems, and their representation on cell surfaces is commonly called
Peptide Modifications: N-Terminal, Internal, and C-Terminal
Peptide Modifications: N-Terminal, Internal, and C-Terminal
Cleavage and Purification of GST-Tagged Protein Bound to GSTrap
This page shows how to cleave and purify GST-tagged proteins bound to GSTrap from Cytiva.
Glycosyltransferases were initially considered to be specific for a single glycosyl donor and acceptor, which led to the one enzyme-one linkage concept. Subsequent observations have refuted the theory of absolute enzymatic specificity by describing the transfer of analogs of some
ChiPros Chiral Amines
Chiral amines play an important role in stereoselective organic synthesis. They are used directly as resolving agents, building blocks, or chiral auxiliaries.
Azide Building Blocks for Chemical Ligation
Chemoselective ligation strategies are a key success factor for chemical biology research. Ligation techniques open pathways to fully synthetic large peptides and even proteins.
Mass Spectrometry
What is Mass Spectrometry or MS? What is it used for and how does it work? Read this detailed article with diagrams and example graphs to understand how to use mass spec in your research.
Chiral Amines in Asymmetric Synthesis
Chiral amines have found widespread application in asymmetric synthesis serving, for instance, as chiral bases in enantioselective deprotonation reactions or being valuable substances for resolving racemic mixtures of acids.
Applications of Y-Shape Peg Derivatives for Drug Delivery
The immune system protects the body from disease by resisting the invasion of foreign molecules into its cells, for example peptides and proteins are hydrolyzed by proteolytic enzymes; nucleic acids are hydrolyzed by nucleases; and most small molecules are eliminated
Degrader Building Blocks for Targeted Protein Degradation
Protein Degrader Building Blocks are a collection of crosslinker-E3 ligand conjugates with a pendant functional group for covalent linkage to a target ligand.
Unnatural Amino Acids for Peptide Synthesis
Unnatural amino acids, the non-proteinogenic amino acids that either occur naturally or are chemically synthesized, are becoming more and more important as tools for modern drug discovery research.
Proline Derivatives and Analogs
Proline analogues are promising candidates for tuning the biological, pharmaceutical, or physicochemical properties of naturally occuring, as well as de novo designed, linear, and, cyclic peptides.
Signal Peptide Optimization: Effect On Recombinant Monoclonal IgG Productivity, Product Quality And Antigen-Binding Affinity
A signal peptide is a 5-30 amino acid (aa) peptide present at the N-terminus of secretory proteins.
GlycoProfile™ ß-Elimination Kit
Our's GlycoProfile Beta-Elimination Kit allows researchers to perform complete glycoproteomics research by preserving both the O-glycans and protein, specifically remove o-glycans, label o-glycans prior to analysis, have confidence in uniformity of procedure. While it is known that O-glycosylation plays
Biotinylated Peptides
Our Streptavidin HC (High-Capacity) multiwell plates are prepared with a highly-porous, high-density streptavidin coating. Streptavidin has similar biotin-binding characteristics as avidin. Streptavidin can bind 4 moles of biotin per mole of protein with high selectivity and affinity (Kd~10-15). Unlike avidin
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