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Amicon® Stirred Cells
User guide for Amicon® Stirred cells including features, operation, specifications, and troubleshooting.
cOmplete™ His-Tag Purification Resin Protocol & Troubleshooting
cOmplete™ His-Tag Purification Resin Protocol & Troubleshooting
Enzymatic Assay of Phytase (EC 3.1.3.8)
Derived from procedure SSPHYT02. Includes template updates to current SOP specifications and incorporation of notes into the procedure.
Enterobacteriaceae in Food
General guidance for the detection and enumeration of Enterobacteriaceae in food according to EN-ISO 8523:1991 and EN-ISO 4832:1991, respectively.
ARGET ATRP: Procedure for PMMA Polymer Brush Growth
We presents an article about ARGET ATRP, and its procedure for PMMA polymer brush growth. Surface preparation before polymer brush growth consists of two steps: surface cleaning and initiator monolayer deposition.
Enzymatic Assay of Alcohol Oxidase (EC 1.1.3.13)
This procedure may be used for all Alcohol Oxidase products. The continuous spectrophotometric rate determination (A405, Light path = 1 cm) is based on the following reactions.
GC Analysis of Oxygen, Nitrogen, and Carbon Dioxide on Carboxen®-1010 PLOT
GC Analysis of Oxygen, Nitrogen, and Carbon Dioxide on Carboxen®-1010 PLOT
CRISPR Cas 9 Nuclease RNA-guided Genome Editing
Learn about CRISPR Cas9, what it is and how it works. CRISPR is a new, affordable genome editing tool enabling access to genome editing for all.
Enzymatic Assay of Esterase
Objective: To standardize a procedure for the enzymatic determination of Esterase activity using Ethyl Butyrate as a substrate.
Enzymatic Assay of Acid Phosphatase (EC 3.1.3.2)
Enzymatic Assay of Acid Phosphatase (EC 3.1.3.2)
Enzymatic Galactose and Lactose Assay Protocol
This assay protocol is suitable for the colorimetric/fluorometric detection of Galactose and Lactose in cell and tissue culture supernatants, urine, plasma, serum, and other biological samples using the Galactose and Lactose Assay Kit (MAK011). In this assay kit, galactose is
Rapid DNA Ligation Kit Protocol
Rapid DNA Ligation Kit Protocol
HPLC Analysis of Aspirin and Salicylic Acid on Ascentis® Express RP-Amide
Separation of Acetylsalicylic acid, analytical standard; Salicylic acid, BioXtra, ≥99.0%
SnAP Reagents
Saturated N-heterocyclic building blocks or SnAP Reagents are of growing importance for the convenient synthesis of medium-ring saturated N-heterocycles, including bicyclic and spirocyclic structures. SnAP reagents are stable and readily available and can be coupled with widely available aromatic, heteroaromatic
Procedure for Enzymatic Assay of α-Chymotrypsin (EC 3.4.21.1)
Follow our procedure for the determination of a chymotrypsin activity. This enzymatic assay of alpha chymotrypsin guides you through the entire process and necessary calculations.
MSTFA/MSTFA-d9 Derivatization of Amphetamine for GC/MS Detection and Identification
Example displays how MSTFA/MSTFA-d9 derivatization of amphetamine provides valuable information in mass spectra and chromatograms using SLB-5ms GC column.
Selecting GC Columns by Industry or Application
We have identified the GC columns that are routinely used in the various industries. These are conveniently arranged by industry, and then application within that industry, to simplify the process of selecting the proper phase.
Duolink® PLA Probemaker Guide
Guide to create custom PLA probes for immunofluorescent or brightfield detection.
Assay Procedure for Protease
This procedure is for informational purposes including assay Procedure, definition, and calculations for Protease
Determination of Water Content in Glycerine, Glycerol, 1,2,3-Propantriol Using Karl Fischer Titration
Summary application report for analysis of moisture in Glycerine, Glycerol, 1,2,3-Propantriol
Enzymatic Method for Determining Galactose (Galactose Assay)
This assay protocol is suitable for the colorimetric/fluorometric detection of Galactose and Lactose in cell and tissue culture supernatants, urine, plasma, serum, and other biological samples using the Galactose Assay Kit.
Performing a Purification of Antibody Fragments with Capto™ L Products
This page shows how to purify antibody fragments by affinity chromatography using Capto L products from Cytiva.
Performing a Purification of IgG Antibodies with Protein G HP SpinTrap™/Ab SpinTrap™
This page shows how to separate IgG antibodies by affinity chromatography using Protein G HP SpinTrap™/Ab SpinTrap™ from Cytiva.
Performing a Purification of IgG Antibodies with Protein G Sepharose® 4 Fast Flow
This page shows how to separate IgG antibodies by affinity chromatography using Protein G Sepharose 4 Fast Flow from Cytiva.
Liberase™ TL Research Grade Protocol & Troubleshooting
Liberase™ TL Research Grade Protocol & Troubleshooting
Nucleic Acid Sample Preparation Methodology
Spectrophotometry can be used to estimate DNA or RNA concentration and to analyze the purity of the preparation.
QuickLink™ DNA Ligation Kit
T4 DNA ligase is used for the joining of DNA molecules with compatible cohesive (sticky) termini, joining of blunt ended double stranded DNA molecules
Protocol for DNA extraction & WGA Amplification from Soil Samples
Genomic DNA from soil samples can be easily damaged by nucleases and contaminating debris resulting in low DNA yields. As a result, the researcher’s ability to perform downstream analysis may be compromised. After isolating DNA from the soil sample, the
GC Analysis of Peppermint Essential Oil on SLB®-IL59
Cymene; 4,5,6,7-Tetrahydro-3,6-dimethylbenzofuran; Linalool; Menthol; Menthone; Menthyl acetate; Germacrene D; Bicyclogermacrene; Thymol
DIG Gel Mobility Shift Assay Troubleshooting
To prevent nonspecific binding of the analyzed protein or extract to the DNA fragment or oligonucleotide, nonspecific competitor nucleic acid is added to the binding reaction.
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