Product No: P6222
Our Precast Agarose Gels for RNA Electrophoresis are suitable for separating RNA sizes from 0.25 to 10 kb. The 8-well gels are 1.25% RNase-free agarose (A9539) prepared using 1X MOPS buffer with no denaturants. The gels are formulated without formaldehyde and are recommended for the analysis of total RNA, in vitro RNA transcripts and Northern blotting.
Denaturation of the RNA is usually not necessary before loading the gel. However, if significant secondary structure of the RNA is suspected, the RNA should be denatured using one of the protocols included in the procedure section.
Our Precast Agarose Gels for RNA electrophoresis are for laboratory use only. Not for drug, household or other uses. Many reagents used in electrophoresis are hazardous. Warning statements are included on the label. Always wear personal protective equipment including gloves and eye protection when handling. Refer to Safety Data Sheet.
Our Precast Agarose Gels for RNA analysis are suitable for use with Bio-Rad Mini-Sub®, Hoefer Minnie the Gel-Cicle® , Life Technologies Horizon® 58 and our submarine mini-gel (E0638) electrophoresis units.
Additional reagents required for specific denaturation methods:
Prepare sample using one of the following procedures:
A. No denaturation required
Bring RNA up to a volume of 8 µl with RNase-free water or 1X MOPS buffer. Add 2 µl of
gel loading solution (G7654).
B. Formamide-only denaturation
Bring RNA up to a volume of 8 µl with RNase-free water. Add 2 µl 10X MOPS buffer and 9 µl deionized
formamide. Mix and heat samples for 10 minutes at 70 °C, then chill on ice for at least one minute.
C. Formaldehyde denaturation
Bring RNA up to a volume of 6 µl with RNase-free water. Add 12 µl RNA sample loading buffer (with or without
ethidium bromide). Mix and heat samples for 10 minutes at 70 °C, then chill on ice for at least one minute.
D. Glyoxal denaturation
Prepare glyoxal/DMSO mixture by mixing 2.5 mL DMSO with 1.5 mL deionized glyoxal and 1 mL 10X MOPS buffer.
Quickly dispense small aliquots into microcentrifuge tubes and store at –80 °C. Thaw each aliquot only once; do
Bring RNA up to a volume of 9 µl with RNase-free water. Add 9 µl glyoxal/DMSO mixture. Mix and heat samples
for 60 minutes at 50 °C, then chill on ice for at least one minute.
Our Precast Agarose Gels for RNA analysis require less than 5 minutes to set up.
1. Peel the paper backing from the adhesive strips on the bottom of the tray.
2. Peel off the lid. Leave the gel in the tray.
3. Press the tray directly onto the chamber platform. Align the wells so the RNA samples will run straight.
4. Pour 1X MOPS running buffer in the chamber to a depth of 5 mm OVER the flange of the tray.
5. Load the RNA sample (≤15 μl volume).
6. Electrophorese the gels at 3.5 V/cm for 2 hours. Lower voltages for longer times are acceptable.
RNA marker template set (R4142)