[Skip to Content](https://www.sigmaaldrich.com#main-content) [![Merck](https://www.sigmaaldrich.com/static/logos/purple/merck.svg)](https://www.sigmaaldrich.com/IL/en) Products Cart0 ILEN Products [Login / Register](https://www.sigmaaldrich.com/oidc-sign-in) [Order Lookup](https://www.sigmaaldrich.com/IL/en/order-lookup) [Quick Order](https://www.sigmaaldrich.com/IL/en/quick-order) Cart0 [Home](https://www.sigmaaldrich.com/IL/en)[Sample Purification](https://www.sigmaaldrich.com/IL/en/applications/analytical-chemistry/sample-preparation/purification)Selection of Purification Equipment # Selection of Purification Equipment Simple IEX chromatography, such as elution by a step-gradient, can be performed using a syringe or peristaltic pump with prepacked HiTrap columns. A chromatography system is required in order to achieve a high resolution separation using accurately controlled linear gradient elution, to take advantage of the high flow rates of modern media, or when the same column is to be used for many runs. Way of workingPilotExplorer 100Purifier 10FPLCPrimeSyringe or peristaltic pump + HiTrap column Simple, step-gradient elution✓✓✓✓✓✓ Reproducible performance for routine separation✓✓✓✓✓ Optimization of one step separation to increase purity✓✓✓✓✓ System control and data handling for regulatory requirements, e.g. GLP✓✓✓✓ Automatic method development and optimization✓✓✓✓ Automatic buffer preparation✓✓✓ Automatic pH scouting✓✓✓ Automatic media or column scouting✓✓ Automatic multi-step purification✓✓ Scale up, process development and transfer to production✓✓ Proven sanitary design for cGMP with easily exchanged wetted parts✓ Standard ÄKTAdesign configurations ![Standard ÄKTAdesign configurations](https://www.sigmaaldrich.com/content/dam/cms-commons/sigmaaldrich/marketing/global/images/technical-documents/articles/analytical-chemistry/purification/akt-design-configurations.jpg "Standard ÄKTAdesign configurations") __Figure 1.__ Standard ÄKTAdesign configurations ## Materials Sorry, an unexpected error has occurred Response not successful: Received status code 500 __Related Articles__ - [Maintenance of Multimodal Chromatography Media and Storage Conditions](https://www.sigmaaldrich.com/IL/en/technical-documents/protocol/protein-biology/protein-purification/maintenance-of-media-and-storage-conditions) - [Bind/Elute vs Flowthrough Mode in Multimodal Chromatography](https://www.sigmaaldrich.com/IL/en/technical-documents/technical-article/analytical-chemistry/purification/bind-elute-vs-flowthrough-mode-in-multimodal-chromatography) - [Chiral Chromatography Frequently Asked Questions](https://www.sigmaaldrich.com/IL/en/technical-documents/technical-article/analytical-chemistry/purification/faq) - [FastWoRX for Faster and Greener Alternative to Liquid-Liquid Extraction](https://www.sigmaaldrich.com/IL/en/technical-documents/technical-article/analytical-chemistry/purification/fastworx) - [High Purity Carbon Adsorbents for Chromatography](https://www.sigmaaldrich.com/IL/en/technical-documents/technical-article/analytical-chemistry/purification/high-purity-carbon-adsorbents) - [Plasmid purification by ion exchange chromatography with Genopure kits](https://www.sigmaaldrich.com/IL/en/technical-documents/technical-article/analytical-chemistry/purification/ion-exchange-chromatography) - [LRA (Lipid Removal Agent) for Biopharmaceutical Production](https://www.sigmaaldrich.com/IL/en/technical-documents/technical-article/analytical-chemistry/purification/lra-lipid-removal) - [Immobilized Metal Chelate Affinity Chromatography (IMAC)](https://www.sigmaaldrich.com/IL/en/technical-documents/technical-article/analytical-chemistry/purification/metal-chelate-afnity-chromatography) - [View More](https://www.sigmaaldrich.com/IL/en/search/facet-search?focus=sitecontent&term=facet-search) Top __Sign In To Continue__ To continue reading please sign in or create an account. Sign In__Don't Have An Account?__Register An unknown error has occured. - English - EN [Learn More](https://www.sigmaaldrich.com)