In 2005, the European Commission (EU) recommended the monitoring of 15 priority PAHs along with an additional PAH highlighted by the Joint FAO/WHO Expert Committee on Food Additives (JECFA) which are considered toxic in food products.1 The list contains eight of the EPA’s priority PAHs along with eight other compounds that are known carcinogens.
In this application, we demonstrate a PAH analysis method for the separation of EU 15 + 1 PAH compounds on a 50 x 4.6 mm, 2.7 μm Ascentis® Express PAH column. The analysis was completed under the given conditions (Table 1) in less than ten minutes with an excellent resolution between the critical pair, peaks 4 and 5 (chrysene and 5-methylchrysene), that only differ by the presence of a methyl group (Figure 1).
Figure 1.Separation of EU 15 + 1 polycyclic aromatic hydrocarbons using an Ascentis® Express PAH HPLC Column (Peak IDs Table 2).
The new Ascentis® Express PAH HPLC column allows a highly efficient separation of 16 PAHs in 10 minutes. The Fused-Core® technology of Ascentis® Express PAH HPLC columns enables fast, efficient, and rugged separations, which are paramount to environmental and food analysis of polycyclic aromatic hydrocarbons (PAHs).
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