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1.05642

HPTLC plates, Silica gel 60 F₂₅₄

pkg of 50 plates, plate L × W 20 cm × 10 cm, glass support

Synonym(s):

HPTLC Silica Gel Plates

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Pack SizeSKUAvailabilityPrice
50 units

Estimated to ship on17 April 2026fromSIAL Bangalore Warehouse

₹95,910.00

About This Item

NACRES:
NB.21
UNSPSC Code:
41115711

₹95,910.00


Estimated to ship on17 April 2026Details


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Quality Level

material

glass support, silica gel 60 matrix

feature

binder Organic Polymer, fluorescent indicator

packaging

pkg of 50 plates

technique(s)

thin layer chromatography (TLC): suitable

layer thickness

200 μm

plate L × W

20 cm × 10 cm

particle size

5-6 μm

pore size

60 Å medium pore diameter

storage temp.

2-30°C

General description

50 Glass plates 20 x 10 cm
HPTLC plates deliver fast and quantitative analysis of complex samples for manual or automated use. Our HPTLC silica plates work three times faster than conventional TLC plates – and they are more sensitive. The particle sizes range in HPTLC is between 4-8 µm, which creates a smoother surface and a higher separation power than TLC plates.

Application

Glass HPTLC Silica gel 60 F₂₅₄ plates. Silica gel HPTLC plates size 20 x 10 cm, 50 sheets.

Analysis Note

Specific surface area (according to BET; 5-Pt. measurement): 480 - 540 m²/g
Pore volume (N₂-isotherm): 0.74 - 0.84 ml/g
d 50 (laser diffraction, size distribution): 5 - 7 µm
Layer thickness: 150 - 200 µm
Deviation of layer thickness per plate: ≤ 35 µm
Chromatographic testings:
A) Colour test
hRf-values
- bleu vif organol, colour test, lipophile:
17 - 22
- Ceres Black G, colour test, lipophile: 38 - 43
- ceres Violet brn, colour test, lipophile: 58 - 64
separation number
- colourtest, lipophile:
≥ 10.0
B) Steroid test
hRf-values
- hydrocortisone, steroid test:
23 - 29
- Reichstein S, steroid test: 36 - 45
- methyltestosterone, steroid test: 43 - 52
separation number
- steroid test:
≥ 10.5
Typical value determined on a conditioned plate
Eluent A) Toluene (45% rel.humidity)
Eluent B) Ethyl acetate/Toluene (95+5 v/v; 20% rel.humidity)

Other Notes

Replaces: 5642-6; 5642

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material

glass support, silica gel 60 matrix

material

glass support, silica gel 60 matrix

material

glass support, silica gel 60 matrix

material

aluminum support, silica gel 60 matrix

technique(s)

thin layer chromatography (TLC): suitable

technique(s)

thin layer chromatography (TLC): suitable

technique(s)

thin layer chromatography (TLC): suitable

technique(s)

thin layer chromatography (TLC): suitable

pore size

60 Å medium pore diameter

pore size

60 Å medium pore diameter

pore size

60 Å medium pore diameter

pore size

60 Å medium pore diameter

feature

binder Organic Polymer, fluorescent indicator

feature

binder Organic Polymer, fluorescent indicator

feature

binder Organic Polymer, fluorescent indicator

feature

binder Organic Polymer, fluorescent indicator

storage temp.

2-30°C

storage temp.

2-30°C

storage temp.

2-30°C

storage temp.

2-30°C

plate L × W

20 cm × 10 cm

plate L × W

10 cm × 10 cm

plate L × W

10 cm × 10 cm

plate L × W

5 cm × 7.5 cm


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Protocols

HPTLC fingerprinting enables rapid identification of passion flower with related reference materials.

Powdered black pepper tested for pierine content follows USP guidelines using HPTLC and HPLC for dietary supplements.

HPTLC was used to analyze caffeine in coffee. Filtration of coffee through a PTFE syringe filter was the only sample preparation. Quantitation was conducted with a TLC scanner.

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Articles

In this article we present several HPTLC applications and analytical standards for ginsenosides.

HPTLC plates provide superior separation, speed, and sensitivity, making them ideal for advanced qualitative and quantitative analysis.

An HPTLC method for rapid urea detection in milk using a TLC plate scanner and Silica Gel 60 F254 HPTLC plate.

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Global Trade Item Number

SKUGTIN
105642000104022536066585

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