17-10086
EZ-Magna ChIP® A/G Chromatin Immunoprecipitation Kit
Single day chromatin immunoprecipitation (ChIP) kit containing all necessary reagents to perform 22 individual chromatin immunoprecipitation (ChIP) reactions using magnetic A/G beads. Control primers included.
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Quality Level
manufacturer/tradename
Magna ChIP®
technique(s)
immunoprecipitation (IP): suitable
shipped in
dry ice
Related Categories
General description
- Magnetic bead-based rapid protocol allows performance of ChIP in as little as 1 day
- Blend of protein A+G bead blend allows ChIP using a broader range of antibodies than A or G alone
- Negative and positive control antibodies and control primer set to simplify validation of experimental procedure
- Includes spin columns to make DNA purification easier and more reliable - no more messy phenol-chloroform extractions
- Complete kit with all required reagents for reliable and reproducible results
- Compatible with ChIPAb+ validated antibody and primer sets
Chromatin Immunoprecipitation (ChIP) is an important technique allowing the analysis of in vivo interactions of proteins with genomic DNA. Any chromatin-associated or DNA binding protein can be analyzed with this technique, provided a good antibody to the protein exists. One can measure different proteins localized to a specific region of the genome, or the genome wide distribution of a specific protein. Another powerful application of this technique is to analyze changes in histone modifications that correlate with processes like transcription, mitosis or DNA repair.
Application
Epigenetics & Nuclear Function
Packaging
Components
ChIP Dilution Buffer
Low Salt Wash Buffer
High Salt Wash Buffer
LiCl Wash Buffer
TE Buffer
Cell Lysis Buffer
Nuclear Lysis Buffer
ChIP Elution Buffer (w/o Proteinase K)
Proteinase K
RNase A
10X Glycine
10X PBS
Protease Inhibitor Cocktail II
Anti-RNA polymerase II, clone CTD4H8
Normal Mouse IgG
Control Primers
Spin Filters
Collection Tubes
Bind Reagent A
Wash Reagent B
Elution Reagent C
Physical form
Storage and Stability
Legal Information
Disclaimer
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Irrit. 2 - Flam. Liq. 2 - Resp. Sens. 1 - Skin Irrit. 2
WGK
WGK 3
Flash Point(F)
55.4 °F
Flash Point(C)
13 °C
Certificates of Analysis (COA)
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Protein and nucleic acid interaction reagents and resources for investing protein-RNA, protein-DNA, and protein-protein interactions and associated applications.
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