This product is a normal mouse serum and may be used as an Fc blocker in flow cytometry applications. For more information, please see: https://www.sigmaaldrich.com/product/Normal-Mouse-Serum,EMD_BIO-NS03L
NS03L
Normal Mouse Serum
This Normal Mouse Serum is validated for use in ELISA, Flow Cytometry, Immunoblotting, Immunofluorescence, Immunohistochemistry, Immunoprecipitation for the detection of Mouse Serum.
Synonym(s):
Mouse Serum
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About This Item
UNSPSC Code:
12352202
NACRES:
NA.43
Biological source:
mouse
Form:
lyophilized
Shipped in:
ambient
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biological source
mouse
form
lyophilized
contains
≤0.1% sodium azide as preservative
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
dilution
(ELISA
Flow Cytometry
Immunoblotting
Immunofluorescence
Immunohistochemistry
Immunoprecipitation )
isotype
IgG
shipped in
ambient
storage temp.
2-8°C
1 of 4
This Item | S5143 | 869019-M | I5381 |
|---|---|---|---|
| form lyophilized | form lyophilized powder | form lyophilized solid | form lyophilized powder |
| biological source mouse | biological source human | biological source - | biological source - |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C | storage temp. 2-8°C |
| shipped in ambient | shipped in - | shipped in - | shipped in - |
| manufacturer/tradename Calbiochem® | manufacturer/tradename - | manufacturer/tradename Calbiochem® | manufacturer/tradename - |
| storage condition OK to freeze | storage condition - | storage condition OK to freeze | storage condition - |
General description
Normal carrier sera from healthy, non-immunized mice.
Application
ELISA (see comments)
Flow Cytometry (see comments)
Immunoblotting (see comments)
Immunofluorescence (see comments)
Immunohistochemistry (see comments)
Immunoprecipitation (see comments)
Flow Cytometry (see comments)
Immunoblotting (see comments)
Immunofluorescence (see comments)
Immunohistochemistry (see comments)
Immunoprecipitation (see comments)
Physical form
Lyophilized serum. Lyophilized from 250 mM sodium chloride, 10 mM sodium phosphate, pH 7.6.
Preparation Note
Reconstitute the lypohilized serum in 1 ml of sterile distilled water with occasional gentle mixing for at least 2 h at 4°C. Following reconstitution, aliquot and freeze (-20°C) for long-term storage or refrigerate (4°C) with 0.1% azide for short-term storage. Avoid freeze/thaw cycles of solutions.
Other Notes
Useful as a blocking agent in immunohistochemical procedures or as a negative control for specific mouse antibodies. For use as a negative control in any application, perform experiments in parallel using primary antibody or normal serum at the same concentration. A signal obtained with the normal serum indicates a nonspecific interaction; any additional signal with the primary antibody indicates a specific antibody-antigen interaction. This antibody has also been reported to work for ELISA, flow cytometry, immunoblotting, immunofluorescence, immunohistochemistry, and immunoprecipitation.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Chronic 3
Storage Class Code
13 - Non Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Is this product can be used as a replacement of Fc blocker in flow cytometry?
1 answer-
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Which strain of mouse is used to derive the serum for product NS03L-1ML?
1 answer-
The serum with catalogue number NS03L-1ML, is pooled from one strain only: Outbred non-swiss white mice, which are also known as Albino mice.
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