B6061

Anti-Beclin 1 (N-terminal) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• Synonym(s): Anti-ATG6, Anti-BECN1, Anti-Coiled-coil myosin-like BCL2-interacting protein, Anti-GT197, Anti-VPS30
• MDL number: MFCD09753851
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: IgG fraction of antiserum
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~60 kDa
• species reactivity: human
• technique(s): immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50-1:100 using biotin/ExtrAvidin^®-Peroxidase staining of heat-retrieved sections of human cerebellum; immunoprecipitation (IP): 1-2 μL using extract of human HEK-293T cells expressing recombinant human Beclin 1 fusion protein; indirect immunofluorescence: suitable; western blot: 1:250-1:500 using whole extract of human HeLa cells
• UniProt accession no.: Q14457
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... BECN1(8678)

Description

General description

Beclin 1 (BECN1) or GT197 is a tumor suppressor gene. It is a Bcl-2 (B-cell lymphoma 2) interacting protein and is the mammalian ortholog of yeast Atg6/Vps30. It is a 450 amino-acid protein having three domains. The gene encoding BECN1 is localized on human chromosome 17q21.

Immunogen

synthetic peptide corresponding to amino acids 29-46 of human Beclin 1, conjugated to KLH via a C-terminal cysteine residue. The corresponding sequence is identical in rat and mouse.

Application

Anti-Beclin 1 (N-terminal) antibody produced in rabbit has been used in:

• immunofluorescent staining
• immunoblotting
• immunohistochemistry
• immunoprecipitation

Yale Center for High Throughput Cell Biology IF-tested antibodies. Each antibody is tested by immunofluorescence against HUVEC cells using the Yale HTCB IF protocol. To learn more about us and Yale Center for High Throughput Cell Biology partnership, visit sigma.com/htcb-if.

Biochem/physiol Actions

Beclin 1 (BECN1) is able to complement the autophagy deficiency of yeast lacking autophagy-related gene 6 (Atg6) and restores autophagy in human MCF7 breast carcinoma cells. Beclin 1 plays a major role in the early development of embryo. It also serves as a haplo-insufficient tumor-suppressor gene. Heterozygous Beclin 1 mutant mice have increased spontaneous tumorigenesis. Beclin 1 is monoallelically deleted in a high percentage of sporadic human breast, ovarian and prostate carcinomas and is expressed at reduced levels in those tumors. In human brains BECN1 decreases in an age-dependent fashion, possibly leading to a reduction of autophagic activity during aging, which may contribute to the accumulation of mutant Huntingtin and the age-delayed disease onset of Huntington disease.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Legal Information

ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves