Skip to Content
Merck

B6429

Casein Blocking Buffer 10x

for Northern and Southern blotting, solution

Synonym(s):

10x Blocking Buffer

Slide 1 of 1
Sign In to View Organizational & Contract Pricing.

Select a Size

500 ML

₹7,620.80

₹7,620.80

Available to ship TODAYDetails

About This Item

NACRES:
NA.54
UNSPSC Code:
12161700

Key Documents

View All Documentation

Skip To

Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

Product Name

Casein Blocking Buffer 10x, for Northern and Southern blotting, solution

grade

Molecular Biology

form

solution

technique(s)

Southern blotting: suitable

storage temp.

room temp

Quality Level

200

Related Categories

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
93017B6185S6639
Casein Blocking Buffer 10x for Northern and Southern blotting, solution

Sigma-Aldrich

B6429

Casein Blocking Buffer 10x

SSC Buffer 20x for Northern and Southern blotting, solution

Sigma-Aldrich

93017

SSC Buffer 20x

Bionic™ Buffer, 10X Concentrate for Northern and Southern blotting, solution

Sigma-Aldrich

B6185

Bionic™ Buffer, 10X Concentrate

SSC Buffer 20× Concentrate for Northern and Southern blotting, solution

Sigma-Aldrich

S6639

SSC Buffer 20× Concentrate

grade

for molecular biology

grade

for molecular biology

grade

for molecular biology

grade

for molecular biology

technique(s)

Southern blotting: suitable

technique(s)

Southern blotting: suitable

technique(s)

-

technique(s)

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

form

solution

form

solution

form

solution

form

solution

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

Application

Casein Blocking Buffer 10x has been used in:

  • western blotting[1][2][3]
  • enzyme linked immunosorbent assay (ELISA)[4]
  • immunohistochemistry[5]

Features and Benefits

  • Better blocking performance than dried milk or BSA
  • Easy-to-use concentrate
  • Compatible with neutral and positively-charged membranes
  • Compatible with chemiluminescent and colorimetric detection assays

General description

Casein is a phosphoprotein found in milk. It is classified into four subtypes, including αS1-, αS2-, β-, and κ-caseins.[6]
Sigma’s 10x Blocking Buffer is a casein-based blocking agent routinely used to reduce non-specific binding of detection reagents[6] to the Northern and Southern Blot membranes.

Preparation Note

Dilute 10x Blocking Buffer with molecular biology grade water before use.

pictograms

Exclamation markEnvironment
GHS07,GHS09

signalword

Warning

Hazard Classifications

Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000500147
0000500147
0000484834
0000484834
0000479651

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Anna Ah-San Tang et al.
Science signaling, 10(505) (2017-11-16)
Some 30 years after its discovery, phage display remains one of the most widely used methods of in vitro selection. Initially developed to revolutionize the generation of therapeutic antibodies, phage display is now the first choice for screening artificial binding
Michelle M Adams et al.
Experimental neurology, 211(1), 141-149 (2008-03-18)
Caloric restriction (CR) is a daily reduction of total caloric intake without a decrease in micronutrients or disproportionate reduction of any one dietary component. CR can increase lifespan reliably in a wide range of species and appears to counteract some
Chadwick M Hales et al.
Proteomics, 16(23), 3042-3053 (2016-10-09)
Despite a key role of amyloid-beta (Aβ) in Alzheimer's disease (AD), mechanisms that link Aβ plaques to tau neurofibrillary tangles and cognitive decline still remain poorly understood. The purpose of this study was to quantify proteins in the sarkosyl-insoluble brain
Tanja Kalic et al.
The journal of allergy and clinical immunology. In practice, 8(9), 3084-3092 (2020-05-12)
Fish collagen is widely used in medicine, cosmetics, and the food industry. However, its clinical relevance as an allergen is not fully appreciated. This is likely due to collagen insolubility in neutral aqueous solutions, leading to low abundance in commercially
Choline availability modulates human neuroblastoma cell proliferation and alters the methylation of the promoter region of the cyclin-dependent kinase inhibitor 3 gene
Niculescu MD, et al.
Journal of Neurochemistry, 89(5), 1252-1259 (2004)
View All Related Papers

Protocols

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

Casein Blocking Buffer Protocol

Protocol for Casein Blocking Buffer in Southern and Western blotting includes troubleshooting for optimizing signal.

Questions

1–7 of 7 Questions  

  1. Hi, how much time can be the casein blocking buffer 10X stored from its opening?

    1 answer

    1. This product does not have an expiration date once opened. The datasheet indicates the following regarding storage and stability:

      Storage/Stability: Store the 10X Casein Blocking Buffer at room temperature. It is also stable when stored at 2–8°C. Casein may precipitate over time, which is normal and should not impact the performance of the 10X Blocking Buffer. If precipitation is observed, resuspension of the solids can be achieved by inverting the bottle several times or by vortexing for a few seconds.

      Helpful?

  2. Hi, would it be possible to know the % of casein in your Casein Blocking Buffer 10X Cat# B6429? Thank you.

    1 answer

    1. The composition of this product is proprietary and cannot be disclosed. However, it can be stated that the product is a blocking reagent derived from bovine milk casein and does not contain any serum albumin.

      Helpful?

  3. whats the percentage casein in 10X of B6429-500ml ?

    1 answer

    1. Unfortunately, the concentration of casein is considered proprietary.

      Helpful?

  4. Will my RNA samples and probes be safe with this buffer if we are using horseradish peroxidase and chemiluminescent detection?

    1 answer

    1. The 10X Casein Blocking Buffer is recommended for both colorimetric and chemiluminescent detection. It is used as a blocking solution for detection, which occurs after hybridization and is suitable for Northern and Southern applications.

      Helpful?

  5. What is the pH? What is the counter ion? Is there saline salt in the preparation (0.15M)?

    1 answer

    1. Unfortunately, the composition of this product is proprietary and can't be disclosed.

      Helpful?

  6. What is the % w/v casein in this solution?

    1 answer

    1. The casein concentration is 1.8% of the total solution.

      Helpful?

  7. What is the solution solvent? What grade of reagent is this in terms of sterility and associated testing?

    1 answer

    1. As mentioned in the product detail page, this product is a molecular biology-grade product. A solution is prepared by diluting the 10x Blocking Buffer with molecular biology-grade water.

      Helpful?

Reviews

No rating value

Active Filters

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service