Upon reconstitution, it is ideal to store in single-use aliquots at –20 °C, as repeated freezing and thawing may compromise stability. The stability upon reconstitution has not been determined.
I4131
IgG from rat serum
reagent grade, ≥95% (SDS-PAGE), essentially salt-free, lyophilized powder
Synonym(s):
Rat IgG
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10 MG
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50 MG
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₹87,168.30
About This Item
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conjugate
unconjugated
grade
reagent grade
Assay
≥95% (SDS-PAGE)
form
essentially salt-free, lyophilized powder
storage temp.
2-8°C
target post-translational modification
unmodified
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This Item | I9140 | I8015 | I5256 |
|---|---|---|---|
| conjugate unconjugated | conjugate unconjugated | conjugate unconjugated | conjugate unconjugated |
| form essentially salt-free, lyophilized powder | form buffered aqueous solution | form buffered aqueous solution | form essentially salt-free, lyophilized powder |
| storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C |
| grade reagent grade | grade technical grade | grade technical grade | grade reagent grade |
| assay ≥95% (SDS-PAGE) | assay ≥80% (SDS-PAGE) | assay ≥80% (SDS-PAGE) | assay ≥95% (SDS-PAGE) |
| target post-translational modification unmodified | target post-translational modification - | target post-translational modification - | target post-translational modification unmodified |
General description
IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids, Immunoglobins have two heavy (H) and two light (L) chains, linked by disulphide linkages. Each light chain comprises one variable N-terminal region and a constant C-terminal region. Heavy chain has one variable N-terminal region and three or four constant (CH1-CH4) C-terminal region.[1] The four classes of IgG can be IgG1, IgG2, IgG3, and IgG4.[2] IgG antibody provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections. Rat IgG is purified from pooled normal rat serum by fractionation and ion-exchange chromatography.
Application
IgG from rat serum has been used:
- in immunolabeling[3]
- in flow cytometry[4]
- in enzyme linked immunosorbent assay[5]
- as a control in neutrophil depletion assay[6]
Biochem/physiol Actions
IgG antibody have enormous therapeutic potential and its fragment crystallizable (Fc) region contributes for the development of therapeutic antibody.[10]
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Articles
Sigma’s Imprint RNA Immunoprecipitation Kit was used to copurify human argonaute 2 (Ago2)-associated RNAs from HeLa cells. MicroRNAs reverse transcribed and quantitating using Mysticq reagents.
Protocols
Procedure and protocol for Anti Ago-RNA Immunoprecipitation from mammalian cells using the RIP kit
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Instructions
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For Rat IgG product 10MG, I want to resuspend in 50mL PBS to have a final concentration of 0.2mg/mL. Can you let me know the storage after reconstitution (temperature and shelf life please)? Also, do you recommend to aliquot in single use? Thank you!
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