The typical molecular weight for these IGG kappas is 22.5 kDa.
I4640
Human IgG4 Kappa, Recombinant Protein
purified immunoglobulin, buffered aqueous solution
Synonym(s):
Human IgG4 Protein
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I4640-1MG
₹33,910.50
About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
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biological source
human
Quality Level
conjugate
unconjugated
antibody form
purified immunoglobulin
Assay
>95% (SDS-PAGE)
form
buffered aqueous solution
shipped in
dry ice
storage temp.
−20°C
1 of 4
This Item | I4765 | I3784 | I5654 |
|---|---|---|---|
| biological source human | biological source human (recombinant) | biological source mouse | biological source human |
| assay >95% (SDS-PAGE) | assay - | assay ≥95% (microfluidic capillary gel electrophoresis) | assay >90% (microfluidic capillary gel electrophoresis) |
| form buffered aqueous solution | form - | form buffered aqueous solution | form - |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in dry ice |
| Quality Level 300 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
General description
IgG antibody subtype is the most abundant serum immunoglobulin of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to the fetus through the placenta and is vital for immune defense of the neonate against infections. IgG4, κ recombinant protein is obtained by expression of a phage library in a human cell line and purified by affinity chromatography.
Application
The purified IgG4, κ may be used as an immunoglobulin calibrator, reference antigen, blocking agent, or coating protein in a variety of immunoassays including ELISA, dot blot immunoblotting, Western immunoblotting, immunodiffusion, immunoelectrophoresis, hemagglutination, and cell-binding assays.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4.
Analysis Note
The purity and identity are determined by immunoelectrophoresis, indirect ELISA, and SDS-PAGE.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Rafael D Melani et al.
Journal of proteome research, 21(1), 274-288 (2021-12-09)
Methods of antibody detection are used to assess exposure or immunity to a pathogen. Here, we present Ig-MS, a novel serological readout that captures the immunoglobulin (Ig) repertoire at molecular resolution, including entire variable regions in Ig light and heavy
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What is the molecular weight of the Human IgG4 Kappa product I4640?
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Could you please provide me with the formulation information for 'I4640'? We have ordered this product for animal injection, and the formulation information will be necessary when it is diluted with injection buffer.
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I4640 is recombinant, purified Human IgG4 Kappa provided in a solution of 0.01 M phosphate-buffered saline, pH 7.4.
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