The purified Mouse IgG is isolated from pooled normal mouse serum by fractionation and ion-exchange chromatography.
I5381
IgG from mouse serum
reagent grade, ≥95% (SDS-PAGE), lyophilized powder
Synonym(s):
Mouse IgG
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1 MG
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About This Item
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conjugate
unconjugated
grade
reagent grade
Assay
≥95% (SDS-PAGE)
form
lyophilized powder
composition
Protein, ≥80%
storage temp.
2-8°C
target post-translational modification
unmodified
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This Item | I8765 | I4131 | I5506 |
|---|---|---|---|
| conjugate unconjugated | conjugate unconjugated | conjugate unconjugated | conjugate unconjugated |
| storage temp. 2-8°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. 2-8°C |
| form lyophilized powder | form buffered aqueous solution | form essentially salt-free, lyophilized powder | form essentially salt-free, lyophilized powder |
| grade reagent grade | grade technical grade | grade reagent grade | grade reagent grade |
| assay ≥95% (SDS-PAGE) | assay ≥80% (SDS-PAGE) | assay ≥95% (SDS-PAGE) | assay ≥95% (SDS-PAGE) |
| composition Protein, ≥80% | composition - | composition - | composition - |
General description
IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections[1]
Mouse IgG is purified from normal mouse serum by fractionation and ion-exchange chromatography.
Mouse IgG is purified from normal mouse serum by fractionation and ion-exchange chromatography.
Application
Purified mouse IgG may be used as a reference antigen, standard, blocking agent, or coating protein in a variety of immunoassays including ELISA, dot immunobinding, Western immunoblotting, immunodiffusion, and immunoelectrophoresis. Other applications include starting materials for the preparation of immunogens and solid phase immunoadsorbents. Mouse IgG was used as standard in ELISA[2] and chromatin immunoprecipitation assay.[3] It was used to coat nano particles in the development of biosensors.[4][5]
Physical form
Lyophilized from 0.01 M phosphate buffer, pH 7.2, with 0.015 M NaCl
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Lonnie J Lucas et al.
Biosensors & bioelectronics, 23(5), 675-681 (2007-09-18)
Double detection of microsphere light scattering and quantum dot emission was demonstrated for lab-on-a-chip immunoassay without using stationary support. We conjugated quantum dots (QDs) onto microspheres to enable multiplex assays as well as to enhance the limit of detection (LOD).
Fluorescent Biorecognition of Gold Nanoparticle-IgG Conjugates Self-Assembled on E-Beam Patterns
Powell T and Yoon JY
Biotechnol. Progress, 22, 106-110 (2006)
Panojot Bifsha et al.
Molecular neurobiology, 54(7), 4921-4935 (2016-08-16)
Dysfunction of midbrain dopaminergic (mDA) neurons is involved in Parkinson's disease (PD) and neuropsychiatric disorders. Pitx3 is expressed in mDA neuron subsets of the substantia nigra compacta (SNc) and of the ventral tegmental area (VTA) that are degeneration-sensitive in PD.
Bin Li et al.
International immunology, 19(7), 825-835 (2007-06-26)
We have found that FOXP3 is an oligomeric component of a large supramolecular complex. Certain FOXP3 mutants with single amino acid deletions in the leucine zipper domain of FOXP3 are associated with the X-linked autoimmunity-allergic dysregulation (XLAAD) and immunodysregulation, polyendocrinopathy
Paurvi Shinde et al.
Journal of leukocyte biology, 102(1), 57-69 (2017-04-23)
LPS is a powerful adjuvant, and although LPS-mediated TLR4 signaling has been exquisitely delineated, the in vivo mechanism of how TLR4 responses impact T cell priming is far less clear. Besides costimulation, TNF and type 1 IFN are dominant cytokines
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Instructions
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Is I5381 from one mouse or several mice?
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Bonjr,Pouvez vous svp me confirmer que la poudre ne contient pas de conservateur (type sodium azide) et que ces igG sont une fois reconstitués suitable pour cell culture ? Est ce que la solution de reconstitution est fournie avec la poudre à part ? Merci
1 answer-
Product I5381 is azide-free. There is no addition or treatment with sodium azide during the lyophilization process.
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