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M8644

Anti-Mouse IgM (μ-chain specific) antibody produced in goat

affinity isolated antibody, lyophilized powder

Synonym(s):

Goat anti-mouse IgM

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About This Item

MDL number:
MFCD00145913
UNSPSC Code:
12352203
NACRES:
NA.46

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biological source

goat

Quality Level

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

lyophilized powder

technique(s)

indirect ELISA: suitable

storage temp.

2-8°C

target post-translational modification

unmodified

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I0759A8786M1397
Anti-Mouse IgM (μ-chain specific) antibody produced in goat affinity isolated antibody, lyophilized powder

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M8644

Anti-Mouse IgM (μ-chain specific) antibody produced in goat

Anti-Human IgM (μ-chain specific) antibody produced in goat affinity isolated antibody, lyophilized powder

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I0759

Anti-Human IgM (μ-chain specific) antibody produced in goat

Anti-Mouse IgM (μ-chain specific)–Peroxidase antibody produced in goat affinity isolated antibody, buffered aqueous solution

Sigma-Aldrich

A8786

Anti-Mouse IgM (μ-chain specific)–Peroxidase antibody produced in goat

Anti-Mouse IgG (γ-chain specific) antibody produced in goat affinity isolated antibody, lyophilized powder

Sigma-Aldrich

M1397

Anti-Mouse IgG (γ-chain specific) antibody produced in goat

technique(s)

indirect ELISA: suitable

technique(s)

Ouchterlony double diffusion: suitable, quantitative precipitin assay: suitable

technique(s)

direct ELISA: 1:10,000

technique(s)

Ouchterlony double diffusion: suitable

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

biological source

goat

biological source

goat

biological source

goat

biological source

goat

conjugate

unconjugated

conjugate

unconjugated

conjugate

peroxidase conjugate

conjugate

unconjugated

form

lyophilized powder

form

lyophilized powder

form

buffered aqueous solution

form

lyophilized powder

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

General description

Mouse serum-derived IgM may be used as a reference antigen, standard, blocking agent, or coating protein in a variety of immunoassays including ELISA, dot immunobinding, Western immunoblotting, immunodiffusion, and immunoelectrophoresis. Other applications include starting materials for the preparation of immunogens and solid phase immunoadsorbents.
Immunoglobulin M (IgM) antibodies appear early in the course of infections. IgM antibodies are responsible for agglutination of red blood cells in mis-matched blood transfusions. The level of IgM may vary with the status of disease or infection.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Chromatin immunoprecipitation (1 paper)
Enzyme-linked immunosorbent assay (1 paper)
Goat polyclonal anti-Mouse IgM (μ-chain specific) antibody may be used to identify and quantitate the level of mouse IgM in vitro and in human serum or other biological fluids by immunochemical or immunohistological techniques.

Biochem/physiol Actions

Goat polyclonal anti-Mouse IgM (μ-chain specific) antibody reacts with the m-chain of mouse IgM. It is s specific for mouse IgM when tested against purified mouse IgA, IgG1, IgG2a, IgG2b, IgG3, and IgM, myeloma proteins.

Physical form

Lyophilized from 0.01 M sodium phosphate, 0.015 M sodium chloride, pH 7.2

Preparation Note

Reconstitute with 0.135 M sodium chloride.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000295505
0000295505
0000254354
SLCN9742
0000254354

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Yasmine Zouggari et al.
Nature medicine, 19(10), 1273-1280 (2013-09-17)
Acute myocardial infarction is a severe ischemic disease responsible for heart failure and sudden death. Here, we show that after acute myocardial infarction in mice, mature B lymphocytes selectively produce Ccl7 and induce Ly6C(hi) monocyte mobilization and recruitment to the
Louis Scheurer et al.
Life science alliance, 4(11) (2021-08-27)
General consensus states that immunoglobulins are exclusively expressed by B lymphocytes to form the first line of defense against common pathogens. Here, we provide compelling evidence for the expression of two heavy chain immunoglobulin genes in subpopulations of neurons in
Andrew Elvington et al.
Journal of immunology (Baltimore, Md. : 1950), 189(9), 4640-4647 (2012-10-03)
There is mounting evidence indicating an important role for complement in the pathogenesis of cerebral ischemia-reperfusion injury, or ischemic stroke. The role of the alternative complement pathway in ischemic stroke has not been investigated, and there is conflicting data on
Dimitrios Tsiantoulas et al.
Scientific reports, 7(1), 3540-3540 (2017-06-16)
Mice lacking secreted IgM (sIgM -/-) antibodies display abnormal splenic B cell development, which results in increased marginal zone and decreased follicular B cell numbers. However, the mechanism by which sIgM exhibit this effect is unknown. Here, we demonstrate that
Jenna K Bursley et al.
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, 145, 111595-111595 (2020-07-24)
Nrf2 is a transcription factor that regulates cytoprotective cellular responses to oxidative and electrophilic stress. Nrf2 is potently activated by the synthetic food additive, tert-butylhydroquinone (tBHQ), which is widely used as a preservative in oils and processed foods. Previously published
View All Related Papers

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