As listed in the 'PROPERTIES' section, this kit is sufficient for approximately 200 tests. Please see the link below to review additional information available in the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/184/250/tp0300bul.pdf
TP0300
Total Protein Kit, Micro Lowry, Peterson′s Modification
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1 KIT
₹25,074.90
₹25,074.90
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About This Item
NACRES:
NA.32
UNSPSC Code:
12352202
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usage
sufficient for ~200 assays
Quality Level
1 of 4
This Item | PE0230 | MOD50 | L3540 |
|---|---|---|---|
| usage sufficient for ~200 assays | usage - | usage sufficient for 50 reactions | usage - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 100 |
Application
Biochem/physiol Actions
An optional deoxycholate-TCA precipitation step permits removal of interfering substances such as Tris, sucrose, EDTA, salts, etc. Absorbance at 500-800 nm (typically read at 750 nm) is used to determine protein concentration from a standard curve.
Features and Benefits
- Complete kit for protein assays
- Most widely cited protein assay
- Less protein to protein variation than other assays
- Contains reagents that can remove interfering substances
General description
This method utilizes sodium dodecylsulfate, included in the Lowry reagent, to facilitate the dissolution of relatively insoluble lipoproteins. An optional deoxycholate-TCA precipitation step permits removal of interfering substances such as Tris, sucrose, EDTA, salts, etc.
signalword
Danger
hcodes
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1 - STOT SE 3
target_organs
Respiratory system
Storage Class
8A - Combustible corrosive hazardous materials
wgk
WGK 3
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Protocols
To determine protein content, the Warburg-Christian method refers to measuring protein samples at 280 nm using a spectrophotometer.
Related Content
Products for traditional and alternative protein quantitation techniques available, including BCA, Bradford, Lowry, and more.
Protein quantification methods, reagents, and immunoassay technology for accurately measuring the protein concentrations in a variety of samples.
Instructions
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