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TB113 First Strand cDNA Synthesis kit
0908 Page 1 of 7
First Strand cDNA Synthesis kit
About the Kits
First Strand cDNA Synthesis Kit 40 rxn 69001-3
Description
The First Strand cDNA Synthesis Kit is designed for the preparation
Application Note - Transcriptor High Fidelity cDNA Synthesis Kit
No.
Transcriptor High Fidelity cDNA kit for 50 reactions, including 10 control reactions 05 081 955 001
Synthesis Kit kit for 100 reactions 05 091 284 001
kit for 200 reactions 05 081 963 001
Application Note - Relative Quantification of Multiple mRNA Targets and Reference Genes in Spinocerebellar Ataxia
No. Pack Size
Transcriptor First Strand cDNA Synthesis Kit 1) 04 379 012 001
04 896 866 001
04 897 030 001
1 Kit (50 reactions)
1 Kit (100 reactions)
1 Kit (200 reactions)
Transcriptor Reverse Transcriptase
Product Information Sheet-05893151001
influence the cDNA synthesis reaction.
Using the RealTime ready Cell Lysis Kit*, a maximum of 10% of the final cDNA synthesis reaction
volume should be lysate.
Setup of the cDNA Synthesis Reaction
Product Information Sheet-11483188001
Strand cDNA Synthesis Kit for RT-PCR (AMV)
sigma-aldrich.com 3
1. General Information
1.1. Contents
Vial / bottle Label Function / description Content
1 First Strand cDNA Synthesis Kit for RT-PCR
Transcriptor Universal cDNA Master
used as template in the cDNA synthesis reaction, the components of the lysis reaction may
negatively influence the cDNA synthesis reaction.
Using the RealTime ready Cell Lysis Kit*, a maximum of 10% of
Product Information Sheet-thificdnaro
fragment.
Temperature for cDNA Synthesis
Fig. 4: Comparison of different temperatures for cDNA synthesis
cDNA synthesis with Transcriptor High Fidelity cDNA Synthesis Kit was performed at +55°C, +50
Flyer - Accurate cDNA levels for unbiased two-step qRT-PCR
Strand
cDNA Synthesis Kit
04 379 012 001
04 896 866 001
04 897 030 001
1 kit (50 reactions, incl. 10 control reactions)
100 reactions
200 reactions
Transcriptor High Fidelity
cDNA Synthesis Kit*
User Guide - 11117831001
by monitoring the cDNA efficiency on certain RNAs by PCR
(see Fig. 2).
Fig. 2: Comparison of cDNA efficiency of the Roche Life Science cDNA Synthesis System and a cDNA
Synthesis Kit of a major competitor
Product Information Sheet-3353621001
included in the kit to verify
performance of the first-strand cDNA synthesis, tailing reaction, and following amplification.
3′ RACE test principle
First-strand cDNA synthesis is initiated at
Product Information Sheet - 11117831001
by monitoring the cDNA efficiency on certain RNAs by PCR
(see Fig. 2).
Fig. 2: Comparison of cDNA efficiency of the Roche Life Science cDNA Synthesis System and a cDNA
Synthesis Kit of a major competitor
Product Information Sheet - 11483188001
1st Strand cDNA Synthesis Kit for RT-PCR
(AMV)
For the first strand synthesis of single-stranded cDNA from RNA for use as a PCR template
Cat. No. 11 483 188 001 y Version 10
1 kit (30 reactions)
Flyer - Transcriptor Universal cDNA Master for qRT-PCR
First Strand
cDNA Synthesis Kit
1 Kit (50 x 20 µl reactions)
1 Kit (100 x 20 µl reactions)
1 Kit (200 x 20 µl reactions)
05 893 151 001
List Price:
Transcriptor Universal
cDNA Master
100
Data Sheet - XSCRT
structures to
improve the first strand cDNA yield. For cDNA fragment longer than 3
kb, do not ignore the denaturation step.
Xscript 1 + DNA-Y cDNA
Synthesis Kit
Product Number: XSCRT
Data Sheet
Application Note - mRNA Capture Kit in cDNA-AFLP
sci.kun.nl
mRNA
mRNA
ss cDNA
ds cDNA
first digestion
second
digestion
biotinylated oligo-dT binding
first-strand cDNA synthesis
second-strand cDNA synthesis
BstYI
BstYI
BstYI
Application Note - Easy and Reliable qRT-PCR Analysis of Total RNA Isolated from Fresh Frozen and FFPE Tissue Samples
MagNA
Lyser
High Pure FFPE
RNA Micro Kit
MagNA Pure 2.0
Transcriptor First
Strand cDNA
Synthesis Kit
Transcriptor First
Strand cDNA
Synthesis Kit
LightCycler® 480
Instrument
and Universal
Product Information Sheet - INSP1
GenElute
Mammalian Total RNA Purification Kit or TRI Reagent
and follow the instructions provided.
II. First Strand cDNA Synthesis using the First
Strand Synthesis Kit
1. Combine the following components
Product Information Sheet - 11062603001
Editorial Changes
Related Products
Product Pack Size Cat. No.
cDNA Synthesis Kit 1 kit 11 117 831 001
Primer for cDNA Synthesis p(dT)15 20 �g 10 814 270 001
Protector RNase
Inhibitor
2,000
Flyer - Gene Expression Analysis in Cancer Research
Transcriptor First Strand
cDNA Synthesis Kit
04 379 012 001
04 896 866 001
04 897 030 001
1 kit (50 reactions)
1 kit (100 reactions)
1 kit (200 reactions)
LightCycler® 480 Probes
Master (2x
Product Information Sheet - 11939823001
15 Titan One Tube RT-PCR Kit
10 sigma-aldrich.com
3. Results
Effect of varying cDNA synthesis reaction temperatures
Figure 1 shows the effect of varying cDNA synthesis reaction temperatures on sensitivity
Product Information Sheet-transrtro
069 001
Transcriptor First Strand
cDNA Synthesis Kit
1 kit, 50 reactions, including 10 control reactions 04 379 012 001
1 kit, 100 reactions 04 896 866 001
1 kit, 200 reactions 04 897 030 001
Primer
RT-PCR Product Listing
First Strand Synthesis Kit 1 kit
Sufficient for 50 reactions
eAMV™ First Strand
Synthesis Kit
Going to greater lengths—up to 14.1 kb
cDNA transcripts
The eAMV First Strand Synthesis Kit is the ideal
The Complete Roche Portfolio
RNA
First Strand cDNA Synthesis Kit for RT-PCR (AMV)
Suitable for use with sequence-specific primers,
poly(dT)15 primers or random primers, the First Strand
cDNA Synthesis Kit is used to generate
Product Information Sheet - ERT-RO
Editorial changes.
5.3. Ordering Information
Product Pack Size Cat. No.
Reagents, kits
Primer for cDNA Synthesis 40 μg 10 814 270 001
Primer “random” 2 mg, 50 A260 units, 1 μmol
400 reactions à
Data Sheet - MIRRT
MystiCq microRNA cDNA Synthesis Kit, microRNAs
are polyadenylated through a poly(A) polymerase
reaction. Then, ReadyScript™ Reverse Transcriptase
and other necessary reagents for cDNA synthesis are
subsequently
Human ESC Germ Layer PCR Kit
all samples
simultaneously prior to cDNA
synthesis.
Synthesize cDNA under the
exact same conditions where
possible. If necessary, clean up
cDNA after synthesis and
standardize concentrations
before
Product Information Sheet - RDRT
™ Direct mRNA Miniprep Kit, Catalog
Number DMN10). Simple “heat-kill” procedures or the
use of inactivating slurry solutions are not compatible
with ReadyScript cDNA Synthesis Mix. Please call
technical
Product Information Sheet-10109118001
x 25 μmol, 100 mM 11 969 064 001
4 x 1,250 μl, 4 x 125 μmol, 100 mM 03 622 614 001
cDNA Synthesis System 1 kit, up to 10 reactions 11 117 831 001
Protector RNase Inhibitor 2,000 U
Product Information Sheet-fssgmmro
procedure, using the cDNA as the starting sample material. Transcriptor First Strand cDNA Synthesis Kit* is
recommended for reverse transcription of RNA into cDNA. Synthesis of cDNA is performed according
Product Information Sheet - GS0010
amplifying
the second-strand cDNA at 65oC
1 2 3 4 51 2 3 4 5
First-strand
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