Protein pulldown

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Performing a Purification of IgG Antibodies with Protein G HP SpinTrap™/Ab SpinTrap™

This page shows how to separate IgG antibodies by afﬁnity chromatography using Protein G HP SpinTrap™/Ab SpinTrap™ from Cytiva.

Imprint® Chromatin Immunoprecipitation Kit (CHP1) Protocol

The Sigma Imprint Chromatin Immunoprecipitation Kit uses a plate based system to allow rapid ChIP assays in a high throughput format

Immunoprecipitation Kit (Protein A) Protocol & Troubleshooting

Performing a Purification of IgG Antibodies with MabSelect Sure

This page shows how to separate IgG antibodies by afﬁnity chromatography using MabSelect Sure from Cytiva.

Sera-Mag™ SpeedBeads Protocol

Sera-Mag SpeedBeads provide fast and convenient protein isolation; suitable for manual or automated magnetic affinity binding in various applications.

Dextran Sulfate

Dextran sulfates as sodium salts are water-soluble and stable, containing approximately 17% sulfur for various applications.

Immunoprecipitation Kit (Protein G) Protocol & Troubleshooting

Purification from Unclariﬁed Cell Lysate using HisTrap™ FF Crude

This page shows how to purify histidine-tagged recombinant proteins from unclariﬁed cell lysate using HisTrap FF crude from Cytiva.

Membrane Scaffold Proteins: Nanodisc Formation

Nanodisc technology aids membrane protein solubilization, overcoming associated challenges in diverse protein classes.

Separation with Cytiva Products (GST Fusion Proteins)

Affinity chromatography tags like GST fusion proteins facilitate separations in various purification products from Cytiva.

Immunoprecipitation Procedure

Techniques for protein antigen molecular weight determination, protein interactions, enzymatic activity, and post-translational modifications.

Dextran

Dextran polymer details: composed mainly of alpha-D-(1-6) linkages with varied branch lengths.

Antibody Purification using Protein A/G/L Agarose

Antibody purification protocols yield preparations containing endogenous IgG alongside specific antibodies.