Merck

C5VP001A

Millipore

Cellicon® Filter Assembly with Durapore® Membrane

filtration area 0.01 m2

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Synonym(s):
Cellicon® Perfusion Filter with Durapore® Membrane

material

PET support
PVDF
polyester support

Quality Level

Agency

meets USP Biological Reactivity Test for class VI plastics (family component materials)

sterility

sterile; γ-irradiated (level of 25-40kGy)

flow rate measuring range

10 L/min (/m2)

parameter

1 psi max. reverse press. exposure at 25 °C
1-2.5 L process volume
15 psi at 25 °C (max. burst pressure)
5 psi max. inlet pressure at 25 °C

filtration area

0.01 m2

membrane filtration area

0.01 m2

pore size

5 μm pore size

storage temp.

15-25°C

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This Item
PXVVPPC50PXHVMPC50PXGVPPC50
sterility

sterile; γ-irradiated (level of 25-40kGy)

sterility

-

sterility

-

sterility

-

parameter

1 psi max. reverse press. exposure at 25 °C, 1-2.5 L process volume, 15 psi at 25 °C (max. burst pressure), 5 psi max. inlet pressure at 25 °C

parameter

0.03–0.05 L/min recirculation rate, 15 mL min. working volume, 2.8 bar max. transmembrane pressure (40 psig), 5.5 bar max. inlet pressure (80 psi) at 50 °C

parameter

0.03–0.05 L/min recirculation rate, 15 mL min. working volume, 2.8 bar max. transmembrane pressure (40 psig), 5.5 bar max. inlet pressure (80 psi) at 50 °C

parameter

0.03–0.05 L/min recirculation rate, 15 mL min. working volume, 2.8 bar max. transmembrane pressure (40 psig), 5.5 bar max. inlet pressure (80 psi) at 50 °C

filtration area

0.01 m2

filtration area

50 cm2

filtration area

50 cm2

filtration area

50 cm2

pore size

5 μm pore size

pore size

0.1 μm nominal pore size, 0.1 μm pore size

pore size

0.45 μm nominal pore size, 0.45 μm pore size

pore size

0.22 μm nominal pore size, 0.22 μm pore size

storage temp.

15-25°C

storage temp.

-

storage temp.

-

storage temp.

-

General description

Housing/Overmold/ Assembly fittings/Single-use pump head: Polypropylene; Feed screen: Polyethylene terephthalate (PET); Polyester; Tubing: AdvantaFlex (Thermoplastic Elastomer), DOW Pharma 65; Feed screen: Polyethylene terephthalate (PET); Polyester; Pressure sensors: Polysulfone; Cable ties: Nylon
The Cellicon® Perfusion Filter is designed to meet your perfused seed train challenges. It is a flat sheet cell retention filter running in tangential flow filtration mode part of a single-use assembly. Perfusion operations deliver high-density cell cultures that alleviate the burden of processing large production bioreactor volumes while increasing manufacturing flexibility. Introducing perfusion to your seed train makes it possible to inoculate a higher quantity of cells for one or more production bioreactors, thus increasing process efficiency.

Application

Microfiltration
Seed Train Perfusion

Features and Benefits

High throughput. Predictable linear scale up from lab to manufacturing scale. Reliable and reproducible performance. Low crossflow and cell shear.

Other Notes

These products are manufactured in a facility which adheres to Good Manufacturing Practices.

Legal Information

CELLICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Durapore is a registered trademark of Merck KGaA, Darmstadt, Germany

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Articles

This page describes the design attributes of the Cellicon® filter assembly for cell retention and how they simplify scale-up of intensified upstream processes.

This page describes the financial and operational advantages offered by the Cellicon® Cell Retention Device in an N-1 perfusion process at the 2,000 L scale in comparison to a cell retention device from another supplier.

How the Cellicon® filter and controller enables seamless adoption of a perfused N-1 seed train step into the cell expansion process.

This application note describes the benefits and performance of Cellvento® 4CHO-X Expansion Medium designed for multiple cell expansion steps from vial thaw to N-1 stages to generate higher biomass for inoculating fed-batch production bioreactors

Related Content

Biopharmaceutical developers need efficient, productive upstream processes that deliver high quality monoclonal antibodies (mAbs) and other types of recombinant proteins. Process intensification can future-proof their manufacturing strategies, reduce costs, and increase facility flexibility.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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