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Merck

D1283

Sigma-Aldrich

Dulbecco′s Phosphate Buffered Saline

With calcium chloride and magnesium chloride, 10×, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

DPBS

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500 ML
€53.80

€53.80

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About This Item

MDL number:
MFCD00131855
UNSPSC Code:
12352207
eCl@ss:
32129211
NACRES:
NA.75

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Quality Level

400

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

components

glucose: no (Dextro)
sodium pyruvate: no

shipped in

ambient

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This Item
D1408D4031D8662
Sigma-Aldrich

Sigma-Aldrich

D1283

Dulbecco′s Phosphate Buffered Saline

Sigma-Aldrich

Sigma-Aldrich

D1408

Dulbecco′s Phosphate Buffered Saline

Dulbecco’s Phosphate Buffered Saline Modified, with 36 mg sodium pyruvate, 50 mg streptomycin sulfate, 100 mg kanamycin monosulfate, 1000 mg glucose/L and CaCl2, liquid, 0.1 μm filtered, suitable for cell culture

Sigma-Aldrich

D4031

Dulbecco′s Phosphate Buffered Saline

Sigma-Aldrich

Sigma-Aldrich

D8662

Dulbecco′s Phosphate Buffered Saline

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

Quality Level

400

Quality Level

400

Quality Level

200

Quality Level

400

form

liquid

form

liquid

form

liquid

form

liquid

sterility

sterile-filtered

sterility

sterile-filtered

sterility

0.1 μm filtered

sterility

sterile-filtered

DPBS, Isotonic saline, cell washing solution, cell suspension solution

DPBS, Isotonic saline, cell washing solution, cell suspension solution

DPBS, Isotonic saline, cell washing solution, cell suspension solution

DPBS, Isotonic saline, cell washing solution, cell suspension solution

components

glucose: no (Dextro)
sodium pyruvate: no

components

sodium pyruvate: no
glucose: no (Dextro)

components

sodium pyruvate: 0.036 g/L
glucose: 1.0 g/L

components

sodium pyruvate: no
glucose: no (Dextro)

General description

Dulbecco′s Phosphate Buffered Saline is an isotonic saline solution used for washing cells and tissues. This balanced salt solution (BSS) is also used in cell culture as a buffering system to maintain the medium within the physiological pH range and as an irrigating, transporting and diluting fluid while maintaining intra- and extracellular osmotic balance.

Application

Dulbecco′s Phosphate Buffered Saline has been used:
  • for decellularization of extracellular matrix (ECM)
  • to rinse biosensor chip to remove physically adsorbed mycolic acids
  • for washing cells before staining during in vitro biocompatibility test

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
RNBM0687
RNBL9909
RNBL1755
RNBK5856
RNBJ5988

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Sarah S Gabriel et al.
Kidney international, 91(4), 842-855 (2017-02-02)
Dent disease is a rare X-linked tubulopathy caused by mutations in the endosomal chloride-proton exchanger (ClC-5) resulting in defective receptor-mediated endocytosis and severe proximal tubule dysfunction. Bone marrow transplantation has recently been shown to preserve kidney function in cystinosis, a
Ho-Ying Wan et al.
Acta biomaterialia, 140, 163-177 (2021-12-08)
The development of bone-like tissues in vitro that exhibit key features similar to those in vivo is needed to produce tissue models for drug screening and the study of bone physiology and disease pathogenesis. Extracellular matrix (ECM) is a predominant
Alba Di Pardo et al.
Cell death & disease, 11(7), 546-546 (2020-07-20)
The longevity-associated variant (LAV) of the bactericidal/permeability-increasing fold-containing family B member 4 (BPIFB4) has been found significantly enriched in long-living individuals. Neuroinflammation is a key player in Huntington's disease (HD), a neurodegenerative disorder caused by neural death due to expanded
Nasim Shahidi Hamedani et al.
Journal of clinical medicine, 10(4) (2021-02-14)
Coagulation factor XIII (FXIII) is a protransglutaminase which plays an important role in clot stabilization and composition by cross-linking the α- and γ-chains of fibrin and increasing the resistance of the clot to mechanical and proteolytic challenges. In this study
Melanie A Burkhardt et al.
Biomaterials science, 5(10), 2009-2023 (2017-08-16)
Blood clots stop bleeding and provide cell-instructive microenvironments. Still, in vitro models used to study implant performance typically neglect any possible interactions of recruited cells with surface-adhering blood clots. Here we study the interaction and synergies of bone marrow derived
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