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MAK490

Sigma-Aldrich

L-Amino Acid Assay Kit

sufficient for 100 colorimetric or fluorometric tests

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Quality Level

application(s)

food and beverages

detection method

colorimetric
fluorometric

relevant disease(s)

genitourinary disorders; cancer

shipped in

wet ice

storage temp.

−20°C

Related Categories

General description

L-amino acids are essential molecules defined by the nature of their side-chain. There are 20 amino acids that are commonly used by most organisms for the production of proteins and other biologically relevant molecules. While amino acids can occur in either the D- or L-enantimer, only the L-form is used by cells.

Features and Benefits

  • Linear detection range: 3.3 to 500 µM (colorimetric assay) and 0.13 to 50 µM (fluorometric assay) for 60 minute reaction.
  • Compatible with high-throughput handling systems.

Suitability

The kit is suitable for L-Amino Acid determination in serum, culture media, tissue homogenates, cell lysates, urine, food samples, etc.

Principle

The L-Amino Acid Assay Kit uses an enzyme-catalyzed oxidation of L-amino acids to convert a dye into a colored or fluorescent form. The absorbance at 570 nm or fluorescence intensity at λEx = 530 nm/λEm = 585 is directly proportional to the L-amino acid concentration in the sample.

Storage Class Code

10 - Combustible liquids

WGK

WGK 2


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