Skip to Content
Merck
  • Bioluminescent liquid light guide pad biosensor for indoor air toxicity monitoring.

Bioluminescent liquid light guide pad biosensor for indoor air toxicity monitoring.

Analytical chemistry (2015-03-17)
Evgeni Eltzov, Avital Cohen, Robert S Marks
ABSTRACT

Indoor air pollution became a recent concern found to be oftentimes worse than outdoor air quality. We developed a tool that is cheap and simple and enables continuous monitoring of air toxicity. It is a biosensor with both a nondisposable (monitor) and disposable (calcium alginate pads with immobilized bacteria) elements. Various parameters to enhance its signal have been tested (including the effect of the pad's orientation, it's exposure to either temperature or time with the air toxicant analyte, and various concentrations thereof). Lastly, the sensor has demonstrated its ability to sense the presence of chemicals in a real, indoor environment. This is the first step in the creation of a sensitive and simple operative tool that may be used in different indoor environments.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Methanol, suitable for NMR (reference standard)
Sigma-Aldrich
Piperidine, ReagentPlus®, 99%
Sigma-Aldrich
Acetone, natural, ≥97%
Sigma-Aldrich
Piperidine, ≥99.5%, purified by redistillation
Sigma-Aldrich
Sodium chloride-35Cl, 99 atom % 35Cl
Sigma-Aldrich
Chloroform, anhydrous, contains amylenes as stabilizer, ≥99%
Sigma-Aldrich
Diethylene glycol, ReagentPlus®, 99%
Sigma-Aldrich
Triphenyl phosphite, 97%
Sigma-Aldrich
Sodium chloride solution, 0.85%
Sigma-Aldrich
Formaldehyde-12C solution, 20% in H2O, 99.9 atom % 12C
Sigma-Aldrich
Sodium chloride, random crystals, 99.9% trace metals basis
Sigma-Aldrich
Acetone, ≥99%, FCC, FG
Sigma-Aldrich
Formaldehyde solution, ACS reagent, 37 wt. % in H2O, contains 10-15% Methanol as stabilizer (to prevent polymerization)
Sigma-Aldrich
Diethylene glycol, puriss. p.a., ≥99.0% (GC), colorless
Sigma-Aldrich
Sodium chloride, tested according to Ph. Eur.
Sigma-Aldrich
Formaldehyde solution, Molecular Biology, BioReagent, ≥36.0% in H2O (T)
Supelco
(±)-Epichlorohydrin, analytical standard
Sigma-Aldrich
Sodium chloride solution, BioUltra, Molecular Biology, ~5 M in H2O
Sigma-Aldrich
Tetraethyl orthosilicate, ≥99.0% (GC)
Supelco
Sodium chloride, reference material for titrimetry, certified by BAM, >99.5%
Supelco
Tetrahydrofuran, analytical standard
Supelco
N,N-Dimethylformamide, analytical standard
Supelco
Methanol, analytical standard
Sigma-Aldrich
Formaldehyde solution, tested according to Ph. Eur.
Supelco
Tetrahydrofuran, Selectophore, ≥99.5%
Sigma-Aldrich
(±)-Epichlorohydrin, purum, ≥99% (GC)
Supelco
Chloroform, analytical standard
Sigma-Aldrich
N-Ethyldiisopropylamine solution, suitable for peptide synthesis, ~2 M in 1-methyl-2-pyrrolidinone
Supelco
Diethyl ether, analytical standard
Sigma-Aldrich
N-Ethyldiisopropylamine, ≥98.0%